Determination of Gastrodin in Gastrodia elata Blume by HPLC with ELSD and DAD

Abstract

Abstract: Anormal phase high performance chromatographic method with evaporative light scattering detector(ELSD) and diode array detector(DAD), using n-hexane/methanol/ethyl acetate(volume ratio 6:3:2 ) as the mobile phase, was established for the direct determination of active constituents-gastrodin(GA) in the ethanol extracts of Gastrodia elata Blume. An silica gel column Zorbax RX-SIL[46 mm (i.d.)×25 cm, 5 μm] was employed. Regression equations revealed the linear relationships( r=0998 and 0999) between the peak areas of GAmeasured by ELSDand DADand its concentration. The recoveries of GAmeasured by ELSDand DADare 104.22% and 97.65%, respectively. The contents of GAmeasured by ELSDand DADare 139 mg/g(2.79% RSD) and 12.1 mg/g(2.99% RSD) respectively in Gastrodia elata Blume. The detection limits of ELSDand DAD( S/N=3) are 3 mg/Land 1 mg/Lrespectively. This method is simple, selective and sensitive.

Key words: High performance liquid chromatography, Evaporative light-scattering detection, Diode array detection, Gastrodia elata Blume, Gastrodin

CLC Number:

O658

TrendMD:

WEI Yang, DING Ming-Yu, LI Hong-Xia . Determination of Gastrodin in Gastrodia elata Blume by HPLC with ELSD and DAD[J]. Chem. J. Chinese Universities, 2001, 22(4): 563.

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Determination of Gastrodin in Gastrodia elata Blume by HPLC with ELSD and DAD

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