Label of AFP Antibody and Electrochemiluminescence Immunoassay

Abstract

Abstract: Anew chemiluminescent reagent isothiocyanato-isoluminol (ITCI) was used to label Anti-AFP. The molar incorporation ratios are estimated to be 0.25～0.50 mole of ITCI per mole Anti-AFP. The results show that there are no apparent changes in the immunoreactivity of the labeled Anti-AFPand the quantum efficiency of ITCIattached to the Anti-AFP. In addition, the conditions of labeling reaction,sandwich immunoassay and electrochemiluminescent reaction were studied. Morever, a new sandwich electrochemiluminescence immunoassay(ECLIA) is firstly established, and used to detect AFP in human serum. The calibration ranges is 5.0～100.0 ng/mLand the minimal detection concentration is 2.0 ng/mL.

Key words: Electrochemiluminescence Immunoassay(ECLIA), Isothiocyanatoisoluminol, α-Fetoprotein, Label

CLC Number:

O657

TrendMD:

ZHUANG Hui-Sheng, WANG Qiong-E, CHEN Guo-Nan, HUANG Jin-Ling, LIN Pei-Cheng . Label of AFP Antibody and Electrochemiluminescence Immunoassay[J]. Chem. J. Chinese Universities, 1999, 20(8): 1194.

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Label of AFP Antibody and Electrochemiluminescence Immunoassay

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