Chem. J. Chinese Universities ›› 2020, Vol. 41 ›› Issue (12): 2658.doi: 10.7503/cjcu20200371

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Proximity Labeling in Cell-cell Interaction Detection

MA Weiyi1, LIU Shibo2, CHEN Peng2()   

  1. 1.Yuanpei College
    2.College of Chemistry and Molecular Engineering,Peking University,Beijing 100871,China
  • Received:2020-06-19 Online:2020-12-10 Published:2020-12-09
  • Contact: CHEN Peng E-mail:pengchen@pku.edu.cn

Abstract:

Cell-cell interactions play an important role in many physiological processes. Elucidating these interactions is current interest in the frontier of interdisciplinary studies integrating of chemistry and life sciences. Enzymatic intercellular proximity labeling has become one of the most promising detection methods for cell-cell interactions, enabled by the rapid advancement of protein engineering techniques, including protein function manipulation, directed evolution of enzymes, and precise spatiotemporal regulation on enzymatic activities. We here describe some of the current proximity labeling designs, highlight key advantages and limitations of each method, and discuss the potential applications of proximity labeling.

Key words: Proximity labeling, Cell-cell interaction, Spatiotemporal resolution

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