Radiolabeling of DNA Bipyramid Nanostructures†

doi:10.7503/cjcu20160407

Abstract

Abstract:

DNA bipyramid nanostructures were radiolabeled with fluorine-18 through click chemistry. Reactant concentration, catalyst dosage, reaction time, temperatureand reaction frequency in the CuAAC reaction were studied. DNA bipyramid nanostructures were also radiolabeled with technetium-99m through stannous chloride lableling method. The results showed that it was not a good idea to radiolabel DNA bipyramid nanostructures with fluorine-18 through click chemistry. At the same time ^99mTc-DBNs were successfully prepared. Radioactive molecular imaging probes would be obtained based on the nanoplatforms of DNA bipyramid nanostructures.

Key words: DNA bipyramid nanostructure, Radiolabeling, Fluorine-18, Technetium-99m

CLC Number:

O621.3

TrendMD:

LI Jianbo, WANG Xuemei, BAO Baoliang, HE Yulin, WANG Xiangcheng, ZHANG Guojian, BAI Xia, LIU Lei. Radiolabeling of DNA Bipyramid Nanostructures^†[J]. Chem. J. Chinese Universities, 2016, 37(9): 1655.

Figures/Tables 6

References 20

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ssDNA	Oligonucleotide sequence
a	AGGCAGTTGAGACGAACATTCCTAAGTCTGAAATTTATCACCCGCCATAGTAGACGTATCACC
b	CTTGCTACACGATTCAGACTTAGGAATGTTCGACATGCGAGGGTCCAATACCGACGATTACAG
c	GGTGATAAAACGTGTAGCAAGCTGTAATCGACGGGAAGAGCATGCCCATCCACTACTATGGCG
A	CTGCGCGGATGACTCAACTGCCTGGTGATACGATCTAGTCTCTACGTCAAGTAAGAACCTTAG
B	CCTCGCATGACATCCGCGCAGCTAAGGTTCAAAGTTCCTGCCGCTTCACGGACGGTATTGGAC
C	CTCTTCCCGACCGTGAAGCGGCAGGAACTTATACTTGACGTAGAGACTAGAAGGATGGGCATG
T20-a*	TTTTTTTTTTTTTTTTTTTTCTAAGTCTGAAATTTATCACCCGCCATAGTAGACGTATCACCAGGCAGTTGAGACGAACATTC
A20	AAAAAAAAAAAAAAAAAAAA

ssDNA	Oligonucleotide sequence
a	AGGCAGTTGAGACGAACATTCCTAAGTCTGAAATTTATCACCCGCCATAGTAGACGTATCACC
b	CTTGCTACACGATTCAGACTTAGGAATGTTCGACATGCGAGGGTCCAATACCGACGATTACAG
c	GGTGATAAAACGTGTAGCAAGCTGTAATCGACGGGAAGAGCATGCCCATCCACTACTATGGCG
A	CTGCGCGGATGACTCAACTGCCTGGTGATACGATCTAGTCTCTACGTCAAGTAAGAACCTTAG
B	CCTCGCATGACATCCGCGCAGCTAAGGTTCAAAGTTCCTGCCGCTTCACGGACGGTATTGGAC
C	CTCTTCCCGACCGTGAAGCGGCAGGAACTTATACTTGACGTAGAGACTAGAAGGATGGGCATG
T20-a*	TTTTTTTTTTTTTTTTTTTTCTAAGTCTGAAATTTATCACCCGCCATAGTAGACGTATCACCAGGCAGTTGAGACGAACATTC
A20	AAAAAAAAAAAAAAAAAAAA

Entry	n(Alkynyl-DBNs)	n(Cu²⁺)	n(TBTA)	n(Sodium ascorbate)
1	1	2.0	0.5	20
2	1	1.0	1.0	10
3	1	0.5	1.0	5
4	1	0.1	2.0	2
5	1	0.1	5.0	5

Entry	n(Alkynyl-DBNs)	n(Cu²⁺)	n(TBTA)	n(Sodium ascorbate)
1	1	2.0	0.5	20
2	1	1.0	1.0	10
3	1	0.5	1.0	5
4	1	0.1	2.0	2
5	1	0.1	5.0	5