High Resolution Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry Analysis of DNA

Abstract

Abstract: High resolution mass spectra of DNAs amples were acquired by using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF-byMS) using mixture of 3-hydroxypiclinic acid and picnilic acid as a matrix. The resolutions of d(C)₁₀, d(G)₁₀, d(T)₁₈, and d(TTACTCTGTTAATGTCTTTG) mono-isotope peaks have reached 5513, 3895, 10468 and 8769 respectively. The experiment results showed that the DNA samples with a higher purity and stability could get higher resolutions. C₁₈Sep-Pak cartridge, NH₄⁺cation-exchange resin and nitrocellulose(NC) film substrate have been applied to eliminating Na⁺, K⁺ions and other impurities from DNA syntheses, and to purifying the analytes and obtaining high resolution mass spectra with enhanced sensitivity and reproducibility.

Key words: Matrix-assisted laser desorption ionization, DNA, High resolution mass spectrometry

CLC Number:

O657.6

TrendMD:

DENG Hui-Min, LI Jun, LAI Zhi-Hui, ZHA Qing-Min, ZHAO Shan-Kai . High Resolution Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry Analysis of DNA[J]. Chem. J. Chinese Universities, 1999, 20(6): 957.

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High Resolution Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry Analysis of DNA

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