Further NMR Investigation and Conformation Study of Polypeptide IS4

Chem. J. Chinese Universities ›› 1999, Vol. 20 ›› Issue (2): 264.

• Articles • Previous Articles Next Articles

Further NMR Investigation and Conformation Study of Polypeptide IS4

TU Guang-Zhong¹, MIAO Zhen-Wei², ZHANG Ri-Qing¹, LUO Xue-Chun JIANG Yun¹, ZHAO Nan-Ming²

1. Biomembrane & Biomembrane Engineering National Laboratory, Department of Biological Science & Biotechnology, Tsinghua University, , Beijing, 100084;
2. College of Chemistry and Molecular Engineering, Peking University, Beijing, 100871

Received:1998-01-19 Online:1999-02-24 Published:1999-02-24

Abstract

Abstract: Two dimensional ¹HNMR technique was used to determine the solution structure of IS4 The crosspeaks volumes in NOESY spectra were used to generate protonproton distance constraints. These data, combined with hydrogen bond constraints and angle constraints, were used to obtain a set of 10 structures by program DIANA. These structures were refined by unrestrained energy minimization using standard minimization program. The results indicated that the conformation of IS4 in CF₃CD₂ODis of helix.

Key words: Polypeptide IS4, 2DNMR, Polypeptide conformation, Structure calculation

CLC Number:

O657

TrendMD:

TU Guang-Zhong, MIAO Zhen-Wei, ZHANG Ri-Qing, LUO Xue-Chun JIANG Yun, ZHAO Nan-Ming . Further NMR Investigation and Conformation Study of Polypeptide IS4[J]. Chem. J. Chinese Universities, 1999, 20(2): 264.

[1]	ZHU Ying, LIU Pei, YANG Xiao-Hai, HE Lei-Liang, LI Qing-Zhao, WANG Qing, WANG Ke-Min, HUANG Jin, LIU Jian-Bo. Single-labeled“Turn on” Fluorescent Oligonucleotide Probe for Iodide Detection Based on T-Hg²⁺-T and Inherent Quenching Ability of G-quadruplex [J]. Chem. J. Chinese Universities, 2012, 33(12): 2651.
[2]	SUN Yang, ZHANG Lei, XU Fei, GONG Bo-Lin. Preparation of Chiral Ligand Exchange Chromatographic Stationary Phase by Atom Transfer Radical Polymerization for Separation of Racemates [J]. Chem. J. Chinese Universities, 2012, 33(12): 2644.
[3]	HE Wei-Na, TU Xi-Juan, GUO Xiang-Qun. Spin-Labeled BSA-modified Near-infrared Fluorescent Gold Nanoparticles [J]. Chem. J. Chinese Universities, 2012, 33(11): 2411.
[4]	YANG Jun, QI Li, MA Hui-Min, CHEN Yi. Fabrication of Nylon Membrane-based Microfluidic Chips and Its Application in Color Sensing of Glucose [J]. Chem. J. Chinese Universities, 2012, 33(11): 2405.
[5]	TIAN Qing-Qing, ZENG Wei-Xiu, ZHAO Xin, HOU Yu-Lan, CHEN Bo, YAO Shou-Zhuo. Solvent Induced Phase Separation Extraction-Spectrophotometric Method for Determination of Angiotensin-converting Enzyme Activity [J]. Chem. J. Chinese Universities, 2012, 33(11): 2417.
[6]	WANG Qing, YANG Li-Yan, LIU Jian-Bo, YANG Xiao-Hai, WANG Ke-Min, JI Hai-Ning, LIU Yan. Dynamic Light Scattering for Fluoride Ions Detection [J]. Chem. J. Chinese Universities, 2012, 33(10): 2195.
[7]	LUO Ya-Hui, LI Gang, CHEN Qiang, ZHAO Jian-Long. Microfluidic Device for Automated High-Throughput Protein Crystallization Screening by Osmotic Dewatering [J]. Chem. J. Chinese Universities, 2012, 33(10): 2178.
[8]	CAO Xia, ZENG Xi, MU Lan, CHEN Yi, WANG Rui-Xiao, ZHANG Yun-Qian, ZHANG Jian-Xin, WEI Gang. Synthesis, Characterization and Aggregation Induced Enhanced Emission of New Phenothiazine Hydrazone [J]. Chem. J. Chinese Universities, 2012, 33(10): 2184.
[9]	HAO Li, CHENG He-Yong, LIU Jin-Hua, YIN Xue-Feng. Direct Determination of Lead and Cadmium in Distilled Spirits by Inductively Coupled Plasma Mass Spectrometry Coupled with Chip-based Microflow Injection System [J]. Chem. J. Chinese Universities, 2012, 33(09): 1957.
[10]	LIU Yue-Qin, PI Zi-Feng, SONG Feng-Rui, LIU Zhi-Qiang, LIU Zhong-Ying. Effect of Acarbose on Urine Metabolic Profiling of Type Ⅱ Diabete Rats [J]. Chem. J. Chinese Universities, 2012, 33(09): 1932.
[11]	FENG Ke-Jun, YAO Yan-Ling, SHEN Guo-Li, YU Ru-Qin. Single-walled Carbon Nanotube/DNAzyme Complex-based Elctrochemical Biosensors [J]. Chem. J. Chinese Universities, 2012, 33(08): 1676.
[12]	WANG Lin, TAN Xue-Cai, ZHAO Dan-Dan, LIU Li, LEI Fu-Hou, HUANG Zai-Yin, GONG Qi. Electrochemical Sensor for Caffeine Detection Based on Molecularly Imprinted Polymer with Maleic Rosin Acrylic Acid Glycol Ester as Cross-linker [J]. Chem. J. Chinese Universities, 2012, 33(08): 1708.
[13]	LIU Xue-Ping, OUYANG Xiang-Yuan, WU Hui-Wang, SHEN Guo-Li, YU Ru-Qin. Novel Fluorescent Technique for Detecting Adenosine Based on DNA Strand Displacement Induced by Target-aptamer Complex and Metal Deposition Catalyzed by Gold Nanoparticles [J]. Chem. J. Chinese Universities, 2012, 33(08): 1692.
[14]	ZHAO Qiang, SUN Xiao-Hong, LU Ying, PAN Ying-Jie, ZHAO Yong. Application of Metabolic Profiling for Distinguishing Different Pathogenic Vibrio Parahaemolyticus [J]. Chem. J. Chinese Universities, 2012, 33(08): 1686.
[15]	YIN Peng-Fei, GONG Hui-Ping, LI Ping-Ping, LIU Zheng-Qing, HE You-Qiu. Fluorescence Spectrometry and UV-Vis Absorption Spectra Analysis of Interaction of Core-shell CdTe/CdS QDs with Palmatine Chloride and Its Applications [J]. Chem. J. Chinese Universities, 2012, 33(07): 1432.

Further NMR Investigation and Conformation Study of Polypeptide IS4

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments