The Process of Conformational Change of Human Hemoglobin Induced by VanadyLIon Binding and the Effect of 2,3-Diphosphoglycerate

Abstract

Abstract: The process of the conformational change of human hemoglobin as the result of its interaction with vanadyLIons with and without DPG was determined by means of stopped flow measurement of the intrinsic fluorescence of Hb. The vanadyl binding causes monotonous quenching of the intrinsic fluorescence of DPG depleted Hb. It is noteworthy that the binding of DPG to Hb makes the process to be biphasic one. The first step is also featured by the quenching of intrinsic fluorescence. In the second step of DPG Hb system, the intrinsic fluorescence stops to decrease and turns to increase very slightly. The process was explained on the basis of vanadylbinding induced conformation change and the stabilizing effect of DPG and its hydrolytic product 3PGA to the tetrameric structure of Hb.

Key words: Vanadyl, Human hemoglobin, 2, 3-Diphosphoglycerate, Conformational change

CLC Number:

O614.5

TrendMD:

XUE De-Ping, LIN Hua-Kuan, WANG Kui . The Process of Conformational Change of Human Hemoglobin Induced by VanadyLIon Binding and the Effect of 2,3-Diphosphoglycerate[J]. Chem. J. Chinese Universities, 1999, 20(2): 167.

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The Process of Conformational Change of Human Hemoglobin Induced by VanadyLIon Binding and the Effect of 2,3-Diphosphoglycerate

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