Determination of Pseudoephedrine in Human Plasma Using Capillary Zone Electrophoresis Coupled by Flow Injection on-line Solid Phase Extraction

Abstract

Abstract: The previously developed hyphenated system of flow injection on-line solid phase extraction-capillary zone electrophoresis(FI-SPE-CZE) was adapted to the determination of trace pseudoephedrine in human plasma. Interference from the coexisting species was eliminated by off-line and on line sample cleaning-up, and by optimization of CZE separation conditions. The sensitivity of hyphenated system was improved by increasing of loaded sample volume and strengthening of field amplified stacking effect. Thus, plasma samples, after deproteinized by off-line precipitation, could be consecutively loaded onto the FI-SPE-CZE system, and trace amounts of pseudoephedrine be automatically preconcentrated, separated and determined by the system. The adapted FI-SPE-CZE system was successfully applied to determination of trace pseudoephedrine in volunteer s plasma samples for pharmacokinetic studies after administration of the drug with normal dosage.

Key words: Capillary electrophoresis, Flow injection, Solid phase extraction, Pseudoephedrine, Plasma

CLC Number:

O657

TrendMD:

CHEN Heng-Wu, FANG Zhao-Lun . Determination of Pseudoephedrine in Human Plasma Using Capillary Zone Electrophoresis Coupled by Flow Injection on-line Solid Phase Extraction[J]. Chem. J. Chinese Universities, 1999, 20(11): 1688.

[1]	QIN Gaizhao, TANG Minghua, LAI Yalin, YUAN Liming. Chiral Metal-organic Cage MOC-PA as a Chiral Stationary Phase for Capillary Electrophoresis [J]. Chem. J. Chinese Universities, 2022, 43(11): 20220417.
[2]	LIU Kun, YIN Yuan, GENG Wenqiang, XIA Haotian, LI Hua. Influence Mechanism of Filling Transition Metal Oxide Catalyst with Different Components on Nitrogen Fixation in Dielectric Barrier Discharge [J]. Chem. J. Chinese Universities, 2022, 43(11): 20220278.
[3]	SHI Ying, HU Guangjian, WU Minjie, LI Feng. Applications of Low Temperature Plasma for the Materials in Li-ion Batteries [J]. Chem. J. Chinese Universities, 2021, 42(5): 1315.
[4]	ZHENG Haijiao, JIANG Liyan, JIA Qiong. Arginine Functionalized Magnetic Nanoparticles and Its Application in Phosphopeptides Enrichment [J]. Chem. J. Chinese Universities, 2021, 42(3): 717.
[5]	WANG Xiaoru,ZHANG Na,XING Jun. Preparation and Application of Melamine Imprinted Material Using Itaconic Acid as Multidentate Functional Monomer [J]. Chem. J. Chinese Universities, 2020, 41(7): 1521.
[6]	WANG Tianqi,YU Qiongwei,FENG Yuqi. Analysis of Imidazole Propionic Acid in Serum of Patients with Type 2 Diabetes Based on NiO@SiO₂ Solid-phase Extraction Coupled with Liquid Chromatography-Mass Spectrometry ^† [J]. Chem. J. Chinese Universities, 2020, 41(2): 262.
[7]	ZHAO Yanfeng,SUN Xiaolong,HU Shaozheng,WANG Hui,WANG Fei,LI Ping. Effect of Oxygen on Photocatalytic Nitrogen Fixation Performance of N Vacancy-embedded Graphitic Carbon Nitride ^† [J]. Chem. J. Chinese Universities, 2020, 41(1): 132.
[8]	HU Xueyi, CHEN Miaomiao, FANG Yun, FENG Ruiqin, HAN Huihui. Investigation on Pseudo-polyanions of Cationic Cellulose-Sodium Dodecylbenzenesulfonate^† [J]. Chem. J. Chinese Universities, 2019, 40(7): 1464.
[9]	MENG Zihui,WANG Yifei,XIE Tengsheng,CHEN Wei,QIU Lili,XUE Min. Molecularly Imprinted Hollow Spheres for the Solid Phase Extraction of Protein^† [J]. Chem. J. Chinese Universities, 2019, 40(1): 62.
[10]	DENG Caisong, PAN Tingting, DU Lan, WANG Ming, NI Haibin, NI Xiaoqi. Fabrication of Monolayer Crystalline Films on Optical Fiber End by Micro-flow Injection Method^† [J]. Chem. J. Chinese Universities, 2018, 39(4): 708.
[11]	YAN Yuwei,JIN Wei,ZHU Dan,ZHANG Tao,YING Yangwei,SHAN Jin,ZHANG Xuchen,YU Bingwen,CHEN Ting,LIU Chao,JIN Qinhan. Application of Kilowatt MPT-AES in Oil Analysis^† [J]. Chem. J. Chinese Universities, 2018, 39(12): 2651.
[12]	ZHANG Luyun, XU Qian, XIA Guangqing, CONG Li, WANG Yu, ZHANG Hui, ZHU Junyi, ZANG Hao. Synthesis and Biological Activity of Lipoic Acid Ester Derivatives^† [J]. Chem. J. Chinese Universities, 2018, 39(10): 2198.
[13]	ZHANG Chanchan, ZHANG Fanghui, DING Lei, NI Zhenjie, JIANG Lang, DONG Huanli, ZHANG Xiaotao, LI Rongjin, HU Wenping. Organic Phototransistor Based on Surface Plasmon Resonance Effect^† [J]. Chem. J. Chinese Universities, 2018, 39(1): 102.
[14]	WANG Xinchen, YANG Meiling, ZHANG Xiaoping. Rapid Qualitative Analysis of Active Molecules in Tablets Using Microwave Plasma Torch Mass Spectrometry by the Regulation of Power^† [J]. Chem. J. Chinese Universities, 2017, 38(4): 561.
[15]	LI Jiahui, ZHANG Qikai, ZHAO Shanlin, LI Ping. Sensitization for Determination of Iron by Microwave Plasma Torch Atomic Emission Spectrometry^† [J]. Chem. J. Chinese Universities, 2017, 38(4): 547.

Determination of Pseudoephedrine in Human Plasma Using Capillary Zone Electrophoresis Coupled by Flow Injection on-line Solid Phase Extraction

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments