Chem. J. Chinese Universities ›› 1998, Vol. 19 ›› Issue (9): 1498.

• Articles • Previous Articles     Next Articles

The Investigation of the Various Structures of DNA Molecules(Ⅲ) The Coil toroid Transition of λ-DNA Induced by the Biomolecular Probe AO

FENG Xi-Zeng, LIN Zhang, MA Jin, WANG Chen, BAI Chun-Li   

  1. Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100080
  • Received:1998-04-28 Online:1998-09-24 Published:1998-09-24

Abstract: The helix and structure transition of λ-DNAin AOsolutions were investigated by SEMand AFMmethods. Using SEM, we observed the turn and globular as well as toroidal condensates of λ-DNAinduced by AO. The fine structures of these condensates were further observed by using AFM. Our results reveal that the mechanism of the coil-toroid transition of λ-DNAresulted from the changed environment is related to the shrinking and collapsing process as well as the self-assembling process of these condensates.

Key words: λ-DNA, AO, Sanning electron microscopy, Atomic force microscopy

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