Chem. J. Chinese Universities ›› 2013, Vol. 34 ›› Issue (9): 2125.doi: 10.7503/cjcu20121137

• Organic Chemistry • Previous Articles     Next Articles

Interaction of Amphotericin B/Sphingomyelin Monolayers at the Air/Water Interface

QIAO Jin-Jing, SUN Run-Guang, HAO Chang-Chun, YANG Jing, MA Xiu-Zi, WANG Xiao-Mei   

  1. Laboratory of Biophysics and Biomedical, College of Physics and Information Technology, Shaanxi Normal University, Xi'an 710062, China
  • Received:2012-12-18 Online:2013-09-10 Published:2013-08-30

Abstract:

In this work, we chose sphingomyelin(SM) as the basic components of the monolayer, which is widespreaded in mammalian cells and takes an important role in biochemical reactions. Different proportion of amphotericin B/sphingomyelin monolayer's surface pressure and mean molecular area(π-A) curve was investigated by the Langmuir-Blodgett(LB) films technology, thermodynamic characteristics were also analyzed based on the π-A curve and the morphological changes of the surface were observed by atomic force microscopy(AFM). The results showed that the compressibility, stability and thermodynamic characteristics of AmB/SM monolayer depended on the mole faction of AmB and the surface pressure obviously. This may be related to the inhibition of Amphotericin B.

Key words: Amphotericin B, Monolayer, Sphingomyelin, π-A Curve, Atomic force microscopy

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