Studies on Immobilization of Urease on Cellulose Acetate Membrane

Abstract

Abstract: Urease was immobilized on cellulose acetate membrane by entrapment. The activity yield and stability of urease immobilized membrane were investigated in catalyzing hydrolysis of urea. The experimental results showed that the activity of urease membrane was related to water content of casting membrane solution. After the enzymatic membrane was used repeatedly for 10 times, the residual activity of urease on the membrane retained 60% of its original. After storage at 4 ℃ for 3 months almost no decrease of enzymatic activity was observed. The optimal pHand temperature of the hydrolysis reaction were also studied. The results showed that this urease immobilized membrane can be used under a wide operational condition.

Key words: Cellulose acetate membrane, Immobilization, Urease

CLC Number:

O621.2

TrendMD:

WANG Jie, SONG Xi-Jin, WU Ming, REN Zhong-Jiao, YUAN Bin, LEI Zhuo-Lin . Studies on Immobilization of Urease on Cellulose Acetate Membrane[J]. Chem. J. Chinese Universities, 1998, 19(7): 1104.

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Studies on Immobilization of Urease on Cellulose Acetate Membrane

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