The ESR Spin Trapping Studies of Reaction Between Ascorbic Acid with Hydrogen Peroxide

Abstract

Abstract: Using 5,5-dimethyl-1-pyrroline N-oxide(DMPO) as a spin-trapping reagent, the oxidation reaction of ascorbic acid was studied. The radical generated in autoxidation process was ascorbate radical anion(A^-·) only. In the presence of hydrogen peroxide, several radical intermediates were found and their structure were determined. The ESR parameters of A^-· and DMPO/radical adducts were measured to be g =2.0056 and α_H=0.17 mT for A^-·, g =2.0059, α_N=1.60 mT and α_H^β=2.28 mT for DMPO/1, g =2.0059, α_N=1.58 mT, α_H^β=2.13 mT and α_H^γ=0.17 mT for DMPO/2, g =2.0059 and α_N= α_H^β=1.49 mT for DMPO/·OH, and g =2.0059 and α_N=1.50 mT for DMPO/3(or 4). The mechanisms for the oxidation reactions of ascorbic acid were proposed on the basis of those observation.

Key words: Ascorbic acid, ESR-spin trapping, Hydrogen peroxide, Ascorbate radical anion

CLC Number:

O621.25

TrendMD:

BAI Ling-Jun, WANG Jian-Ying . The ESR Spin Trapping Studies of Reaction Between Ascorbic Acid with Hydrogen Peroxide[J]. Chem. J. Chinese Universities, 1998, 19(6): 890.

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The ESR Spin Trapping Studies of Reaction Between Ascorbic Acid with Hydrogen Peroxide

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