Investigation on the Factors Affecting Hydrogen Peroxide Concentrations in Natural Biofilm Systems†

doi:10.7503/cjcu20150102

Abstract

Abstract:

In this study, hydrogen peroxide(H₂O₂) was selected as a representative of reactive oxygen species(ROS) to investigate the effect of factors, such as light/dark variation, dark pretreatment of biofilms, biofilm quantity, presence of ligands and pH value of the solutions, on H₂O₂ concentrations in natural biofilms-water systems. The results indicated that H₂O₂ concentration increased rapidly under illumination, and the H₂O₂ concentration decreased(to about 2/3) when the systems switched to darkness. Dark pretreatment signi-ficantly reduced both the H₂O₂ concentrations and the increasing rate. The increase and decrease of biofilm quantity led to corresponding change of H₂O₂ concentrations in the systems. The presence of organic ligands reduced the H₂O₂ concentrations(by about 1/2—1/3). H₂O₂ concentrations in neutral and acidic systems(pH=7 and 5) were about 1.5 times as high as that in basic system(pH=9). These factors affected H₂O₂ concentrations in the biofilm systems by affecting three key processes, which were H₂O₂ generation of natural biofilms, H₂O₂ decomposition and H₂O₂ removal by biofilms.

Key words: Natural biofilm, Hydrogen peroxide, Affecting factor, Visible light illumination, Photosynthesis

CLC Number:

TrendMD:

DONG Deming, SUN Jiaqian, ZHAO Tianyu, LI Ming, HUA Xiuyi, LIANG Dapeng, GUO Zhiyong. Investigation on the Factors Affecting Hydrogen Peroxide Concentrations in Natural Biofilm Systems^†[J]. Chem. J. Chinese Universities, 2015, 36(7): 1337.

Figures/Tables 5

Fig.1 Effect of light/dark variation on H2O2 concentrations in the biofilm systems(A) and H2O2 concentrations in the H2O2 solutions with and without light(B)(n=3)(B) Initial H2O2 concentrations: a, b. 60 μmol/L; c, d. 40 μmol/L; e, f. 20 μmol/L. a, c, e. in darkness; b, d, f. with light.

Fig.2 Effect of dark pretreatment on H2O2 concentrations in the biofilm systems with(A) and without(B) light(n=3)Dark pretreatment time/h: a. 0; b. 12; c. 24.

Fig.3 Effect of biofilm quantity variation(A) and addition of H2O2(B) on H2O2 concentrations in the biofilm systems with light(n=3)(A) a. 4+4 bioflims; b. 4+2 biofilms; c. 4 biofilms; d. 4-2 biofilms; e. 4-4 biofilms. (B) a. 2 biofilms+H2O2; b. 2-2 biofilms+H2O2; c. 4 biofilms; d. 2+2 bioflims.

Fig.4 Effect of organic ligands on H2O2 concentrations in the biofilm systems(A) and H2O2 solutions(B)(n=3) a. Biofilms; b, c and d. biofilms with TA, EDTA and DTPA, respectively.

Fig.5 Effect of pH value on H2O2 concentrations in the biofilm systems(A) and in the H2O2 solutions(B) with light(n=3)pH: a. 5; b. 7; c. 9.

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