The Spectrophotometric Determination of Proteins Using Zincon

Abstract

Abstract: When BSA was added into zincon solution at pH 4.1, the purple colour was observed, which indicates the formation of zincon-BSA complex. The maximum absorption of complex was 564 nm and 594 nm with the apparent molar absorptivity of 1.5×10⁶ L·mol^-1 · cm^-1 . The linear range of standard curve for BSAis 0～10 μg/mL, and the detection limit is 0.5 μg/mL. The basic reaction conditions were investigated. This proposed method was simple, sensitive, and reproducible. The analytical results of human serum albumin using this method is very satisfactory .

Key words: Protein, Zincon, Spectrophotometry

CLC Number:

O657.31

TrendMD:

CHI Yan-Hua, LI Na, ZHUANG Jia, LI Ke-An, TONG Sheng-Yang . The Spectrophotometric Determination of Proteins Using Zincon[J]. Chem. J. Chinese Universities, 1998, 19(6): 879.

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The Spectrophotometric Determination of Proteins Using Zincon

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