A Preliminary Study on Expression of Fibroin C Subunit as a Fusion Protein in E. Coli

Abstract

Abstract: The structural gene of fibroin Csubunit(i.e. fibroin light chain, FL) was amplified by polymerase chain reaction(PCR) from pFL18 plasmid, which carries a cDNAclone of fibroin Csubunit. The PCR product was cloned on fusion protein expression vector pRIT2T, and was expressed in the E. Coli strain P2392. SDS-polyacrylamide gel electrophoresis(SDS-PAGE) and immunoblotting were carried out to verify the expression of protein Afibroin Csubunit fusion protein.

Key words: Fibroin C subunit, Fusion protein, Protein A, Immunoblotting

CLC Number:

Q551

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LI Hong-Jie, CAI Zai-Sheng, SHI Hua-Fang, YU Tong-Yin, HUANG Wei-Da  . A Preliminary Study on Expression of Fibroin C Subunit as a Fusion Protein in E_. Coli[J]. Chem. J. Chinese Universities, 1998, 19(4): 564.

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A Preliminary Study on Expression of Fibroin C Subunit as a Fusion Protein in E_. Coli

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