Purification of Recombinant Human Interferon-γ by High-Performance Hydrophobic Interaction Chromatography and Size Exclusion Chromatography

Abstract

Abstract: Adownstream purification procedure for recombinant human interferon-γ(rhIFN-γ) expressed in E. coli was described. An essentially two-step chromatographic purification procedure, i. e., size exclusion chromatography(SEC) and high-performance hydrophobic in-teraction chromatography (HPHIC), was used for purification of homogeneity of rhIFN-γ from the inclusion body. The specific activity of purified rhIFN-Ywas 1.0×108 IU/mg pro-tein. The product of purification rhIFN-γ was analyzed by an analytical high-per formance SECand a significant sigle symmetrical peak had been found. The purity of purified rhIFN-Ywas greater than 95% from analysis, determination by analytical HPSECand SDS-PAGEwith Coomssie Blue. The molecular weight was ca. 15000. It was shown that this procedure was an effective method for purifying rhIFN-γ.

Key words: High-performance hydrophobic interaction chromatography, Recombinant human interferon-γ, Purification, Size exclusion chromatography

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GUO Li-An, LIU Shu-Lin, ZHU Bao-Quan. Purification of Recombinant Human Interferon-γ by High-Performance Hydrophobic Interaction Chromatography and Size Exclusion Chromatography[J]. Chem. J. Chinese Universities, 1997, 18(6): 920.

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Purification of Recombinant Human Interferon-γ by High-Performance Hydrophobic Interaction Chromatography and Size Exclusion Chromatography

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