Spectroscopic Studies on the Interactions of Water-soluble Porphyrins with Nucleic Acids

Abstract

Abstract: This paper presents the spectroscopic features of the interactions of two por-phyrins, meso-tetrakis (4-trimethylaminoniumphenyl )porphine (TAPP) and meso-tetrakls (N-methyl-4-pyridiniumyl)porphyrin (TMpyP-4 ) with nucleic aclds.Spectra involve electron ab-sorption, fluorescence and resonance light-scattering.The studies of absorption and fluores-cence show that the two porphyrins share common features when they interact with nucleicacids.That is, when the molar ratlo of porphyrins to nuclelc acids, R>0.25, nuclelc acidshave hypochromicity and fluorescence quenching effect on the Soret bands of the two por-phyrins;when R<0.25, new fluorescent products are formed.The difference of the interac-tions of the two porphyrins with nucleic acids can be distingushed by resonance light-scatter-ing spectra.Nucleic acids can strongly enhance the intensity of resonance light-scattering ofTAPP, but not of TMpyP-4.

Key words: meso-Tetrakis(4-trimethylaminoniumphenyl)porphine, meso-Tetrakis(N-methyl-4-pyridiniumyl)porphyrin, Nucleic acids, Fluorescence spectroscopy, Resonance light-scat-tering spectroscopy

TrendMD:

HUANG Cheng-Zhi, LI Ke-An, TONG Shen-Yang. Spectroscopic Studies on the Interactions of Water-soluble Porphyrins with Nucleic Acids[J]. Chem. J. Chinese Universities, 1997, 18(4): 525.

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Spectroscopic Studies on the Interactions of Water-soluble Porphyrins with Nucleic Acids

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