Studies on Kinetic Method for Detecting Active Intermediate in Enzyme Reaction

Abstract

Abstract: Anew kinetic method is proposed for detecting active intermediate in enzyme reaction.Due to the reactivity of the intermediate, a proper competition reagent which reactswith the intermediate while the intermediate changes to final product, can be found andadded.By detecting the klnetic process of intermediate reaction with reagent, the information of intermediate can be obtained.The method can be used to study free radical intermediate and non-free-radical intermediate, to study fast reaction and to follow transient process.With this method, the intermediate process in oxidation reaction of norepinephrine with hydrogen peroxide, catalyzed by horseradish peroxidase, is investigated.

Key words: Kinetic method, Intermediate, Horseradish peroxidase, Norepinephrine

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CAl Ru-Xiu, WU Xin-Guo, LIN Zhi-Xin, CHENG Jie-Ke. Studies on Kinetic Method for Detecting Active Intermediate in Enzyme Reaction[J]. Chem. J. Chinese Universities, 1997, 18(3): 364.

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Studies on Kinetic Method for Detecting Active Intermediate in Enzyme Reaction

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