The Application of an NAD-Silica Biospecific Affinity Chromatography Stationary Phase in Analysis of Nucleotides

Chem. J. Chinese Universities ›› 1995, Vol. 16 ›› Issue (8): 1212.

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The Application of an NAD-Silica Biospecific Affinity Chromatography Stationary Phase in Analysis of Nucleotides

YU Shi-Lin, MIAO Feng-Qin, YANG Zhao-Xia, FENG Ru

Dept.of Appl. Chem., Beijing University of Chemical Technology, 100029

Received:1994-08-18 Revised:1995-01-24 Online:1995-08-24 Published:1995-08-24

Abstract

Abstract: By using a wide-pore(30 nm),microspheric silica(8μm)as the matrix and γaminopropyltriethoxysilane as the coupling agent,a stationary phase of biospecific affinity chromatography was prepared by chemical binding nicotinamide adenine dinucleotide(NAD) to the γ-aminopropyl modified silica. Using the phosphate buffer(pH=7.0)as a mobile phase,the separation of some nucleotides is accomplished by the detection of UVD(260 nm).The effect of changed ion strengh of eluent on the resolution of nucleotide(AMP, ADP and ATP)has been studied.

Key words: NAD, Affinity chromatography, Nucleotide

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YU Shi-Lin, MIAO Feng-Qin, YANG Zhao-Xia, FENG Ru. The Application of an NAD-Silica Biospecific Affinity Chromatography Stationary Phase in Analysis of Nucleotides[J]. Chem. J. Chinese Universities, 1995, 16(8): 1212.

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The Application of an NAD-Silica Biospecific Affinity Chromatography Stationary Phase in Analysis of Nucleotides

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