Studies on the Enzymological Properties of Antibody Induced by N-hydroxymethylmesohemin

Chem. J. Chinese Universities ›› 1995, Vol. 16 ›› Issue (4): 549.

• Articles • Previous Articles Next Articles

Studies on the Enzymological Properties of Antibody Induced by N-hydroxymethylmesohemin

FENG Yan¹, GAO Shu-Juan¹, GAO Gui¹, LIU Xi-Yu¹, YANG Tong-Shu¹, LIU Zi²

1. State Key Lab.of Enzyme Engineering, Jilin University, Changchun, 130023;
2. Institute of Agriculture and Animal Science of PLA of China, Changchun

Received:1994-05-12 Revised:1994-11-30 Online:1995-04-24 Published:1995-04-24

Abstract

Abstract: N-hydroxymethylmesohemin was used as haptin to elicit monoclonal antibody 11D₁through the immunization and cell fusion techniques.The antibody had the ability to combine with mesohemin and showed the activity of peroxidase.Further investigations demonstrate that the affinities of antibody for haptin and mesohemin were 1.1×10^-6and 2.1×10^-6mol/Lrespectively. When antibody-mesohemin complex catalyzed reaction, its K_mand K_catfor H₂O₂were 13. 0 mmol/Land 86 min^-1respectively. Compared with the noncat alyzed reaction, the activity of antibody-mesohemin complex elevated 1.8× 10⁴(mol/L)^-1.

Key words: N-hydroxymethylmesohemin, Abzyme, Peroxidase

TrendMD:

FENG Yan, GAO Shu-Juan, GAO Gui, LIU Xi-Yu, YANG Tong-Shu, LIU Zi. Studies on the Enzymological Properties of Antibody Induced by N-hydroxymethylmesohemin[J]. Chem. J. Chinese Universities, 1995, 16(4): 549.

References

Related Articles 15

[1]	ZHU Haotian, JIN Meixiu, TANG Wensi, SU Fang, LI Yangguang. Properties of Transition Metal-biimidazole-Dawson-type Tungstophosphate Hybrid Compounds as Supports for Enzyme Immobilization [J]. Chem. J. Chinese Universities, 2022, 43(11): 20220328.
[2]	GAO Xia,PAN Huibin,QIAO Chengfang,CHEN Fengying,ZHOU Yuan,YANG Wenhua. Construction of HRP Immobilized Enzyme Reactor Based on Hierarchically Porous Metal-organic Framework and Its Dye Degradation Application^† [J]. Chem. J. Chinese Universities, 2020, 41(7): 1591.
[3]	WANG Ruixue, YIN Dongmei, SONG Yongxin, SHAN Guiye. Preparation of CuS/Ag₂S Nanocomposite and the Peroxidase-like Properties ^† [J]. Chem. J. Chinese Universities, 2020, 41(6): 1218.
[4]	WANG Qishun, ZHANG Zeshu, WANG Huan, LIU Yu, SONG Shuyan, ZHANG Hongjie. Synthesis and Enzymatic Activity of Ni, Ru Co-doped CePO₄ Nanomaterials ^† [J]. Chem. J. Chinese Universities, 2020, 41(5): 947.
[5]	TENG Yu,YANG Shaoming,BAI Chaopeng,ZHANG Jian. Preparation of HRP Molecularly Imprinted Electrochemical Sensor with Multi-walled Carbon Nanotubes as Sensitizing Materials and the Detection of H₂O₂ ^† [J]. Chem. J. Chinese Universities, 2020, 41(1): 78.
[6]	SONG Yichao, HU Mancheng, LI Shuni, ZHAI Quanguo, JIANG Yucheng. Construction of CPO Immobilized Enzyme Reactor Based on Center-radial Dendritic Mesoporous Silica and Its Application ^† [J]. Chem. J. Chinese Universities, 2019, 40(9): 1805.
[7]	CHEN Yan,DONG Xuejiao,SHAN Guiye. Preparation of Liposome@Ag/Au Nanocomposites and Their Interaction with H₂ $O 2 †$ [J]. Chem. J. Chinese Universities, 2019, 40(4): 639.
[8]	LIU Ye, YAO Shunyu, FANG Chao, ZHAO Waiou, WANG Jingyuan, LI Yapeng. Synthesis and Characteristic of Double Model Molecule Probe Targeting Myeloperoxidase [J]. Chem. J. Chinese Universities, 2018, 39(7): 1573.
[9]	GAO Fengqin,WANG Shan,WANG Yunfang,ZHAO Danlei,CUI Ru,JIANG Yucheng. Immobilization of Chloroperoxidase with Magnetic Graphene Oxide and Its Application of Decolorization of Acid Blue 45^† [J]. Chem. J. Chinese Universities, 2018, 39(5): 904.
[10]	WANG Fengjiao, LI Xuelian, HU Mancheng, LI Shuni, ZHAI Quanguo, JIANG Yucheng. Cascade Biodegradation of Organic Pesticide Isoproturon [J]. Chem. J. Chinese Universities, 2017, 38(8): 1362.
[11]	WANG Shengjie, LIU Lixia, JIANG Yucheng, HU Mancheng, LI Shuni, ZHAI Quanguo. Enzymatic Polymerization of Phenols Catalyzed by Chloroperoxidase in the Presence of Ionic Liquids/Quaternary Ammonium Salts^† [J]. Chem. J. Chinese Universities, 2016, 37(9): 1733.
[12]	YIN Juxin, LI Zuhong, MU Ying, LÜ Shaowu, LUO Guimin. Tripeptide Containing Selenium with Glutathione Peroxidase Activity^† [J]. Chem. J. Chinese Universities, 2016, 37(7): 1302.
[13]	FAN Hongliang, ZHANG Tao, JIN Wei, JIN Qinhan. Novel Label-free Assay of Telomerase Based on DNAzyme^† [J]. Chem. J. Chinese Universities, 2015, 36(4): 625.
[14]	ZHOU Haifeng, YANG Dongjie, QIU Xueqing, WU Xiaolei. Structural Characterization, Adsorption and Dispersion Properties of Sodium Lignosulfonate by Horseradish Peroxidase Incubation^† [J]. Chem. J. Chinese Universities, 2014, 35(4): 895.
[15]	GUO Xiao, YU Yang, CHEN Long, WANG Mingshuo, JIN Mengmeng, LIU Guangna, ZHANG Li, LI Tao, GAO Zhuang, WEI Jingyan. Catalytic Activity of Seleno-hGSTZ1c-1c Based on Site-directed Mutagenesis^† [J]. Chem. J. Chinese Universities, 2014, 35(10): 2109.

Studies on the Enzymological Properties of Antibody Induced by N-hydroxymethylmesohemin

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments