Tyrosinase Amperometric Biosensor for Monitoring Inhibitor of Low Concentration of Cyanide

Chem. J. Chinese Universities ›› 1995, Vol. 16 ›› Issue (3): 359.

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Tyrosinase Amperometric Biosensor for Monitoring Inhibitor of Low Concentration of Cyanide

HU Xiao-Ya, LENG Zong-Zhou

Department of Chemistry and Chemical Engineering, Teachers College, Yangzhou University, Yangzhou 225002

Received:1994-04-09 Revised:1994-08-16 Online:1995-03-24 Published:1995-03-24

Abstract

Abstract: Cyandide inhibitor of tyrosinase was determied with biosensor of tyrosinase by using tyrosinase immobilized on a preactivated polyaminde membrane.Phenol with the range of concentration 5×10^-6～4×10^-5mol/Lwas chosen as substrate in phosphate buffer.The de-crease of substrate response have linear relation with square root of concentration of CN-with detection range of 5×10^-7～2×10^-4mol/L. The fine selectivity and reproducibility were obtained.

Key words: Electrode, Biosensor, Enzyme, Tyrosinase, Cyanide

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HU Xiao-Ya, LENG Zong-Zhou . Tyrosinase Amperometric Biosensor for Monitoring Inhibitor of Low Concentration of Cyanide[J]. Chem. J. Chinese Universities, 1995, 16(3): 359.

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Tyrosinase Amperometric Biosensor for Monitoring Inhibitor of Low Concentration of Cyanide

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