The Determination of Anti-MV Antibody in Serum with CL-ELISA

Chem. J. Chinese Universities ›› 1994, Vol. 15 ›› Issue (4): 515.

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The Determination of Anti-MV Antibody in Serum with CL-ELISA

ZHANG Zhu-Jun¹, ZOU Ke-Wei¹, CHENG Ming-Jie²

1. Institute of Analytical science, Shaanxi Normal University, Xian, 710062;
2. Shaaanxi Sanitation and Antiepidemic Station

Received:1993-05-10 Revised:1993-09-20 Online:1994-04-24 Published:1994-04-24

Abstract

Abstract: A method for determination of the anti-MVantibody in serum is established based on chemiluminescence enzyme-linked immunosorbant assay (CL-ELISA).The MV antigen is coated on the polystyrene cup, then reacts with anti-MV serum to form the immunocompound.The anti-HuIgGlabeled by HRPis used as a second antibody.The enzymatic activity on the antibody is determined by p-iodophenol enhanced luminol-H₂O₂ chemiluminescent system, which has higher sensitivity than current chromogenic reaction.The curve of anti-MV antibody title determined with CL-ELISA and ELISA is established and 30 clinical samples are tested.The results show that the CL-ELISAhas higher positive ratio than ELISA.Two methods have good correlation.The correlation coefficient is 0.992.

Key words: Anti-MV antibody, Chemiluminescence, Enzyme Immunoassay, HRP

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ZHANG Zhu-Jun, ZOU Ke-Wei, CHENG Ming-Jie. The Determination of Anti-MV Antibody in Serum with CL-ELISA[J]. Chem. J. Chinese Universities, 1994, 15(4): 515.

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The Determination of Anti-MV Antibody in Serum with CL-ELISA

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