The Influence of Guanidine Hydrochloride on the Retention of Proteins in Hydrophobic Interaction Chromatography

Abstract

Abstract: The retention behavior of five proteins in high performance hydrophobic interaction chromatography(HPHIC)under the mobile phase consisting of(NH₄)₂SO₄-KH₂PO₄ containing guanidine hydrochloride was investigated.By using the stoichiometric displacement model for retention(SDM-R)of solute in liquid chromatography, and the measuring surface tension of the mobile phase in the presence of GuHCl, also and UVspectra, it was found that in the range of low concentraction of GuHCl, the hydrophobic irnteraction is considered as the main contribution to the retention of the proteins, However, whem the concentration of GuHCl in the mobile phase is getting higher, the changes of the molecular conformation of proteins by means of GuHCl in the HPHICbecome the most important factor to influecing the retention of proteins.

Key words: Protein, Hydrophobic interaction chromatography, Guanidine hydrochloride, Changes of molecular conformation

TrendMD:

FENG Wen-Ke, JIANG Xin-Qi, GENG Xin-Du. The Influence of Guanidine Hydrochloride on the Retention of Proteins in Hydrophobic Interaction Chromatography[J]. Chem. J. Chinese Universities, 1994, 15(10): 1450.

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The Influence of Guanidine Hydrochloride on the Retention of Proteins in Hydrophobic Interaction Chromatography

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