Chem. J. Chinese Universities ›› 1993, Vol. 14 ›› Issue (1): 144.

• Articles • Previous Articles    

Synthesis and Kinetics of Chromogenic Substrates for Chymotrypsin

ZHANG Ruo-Heng, GE Xue, XU Xiao-Jie, TANG You-qi   

  1. Deportmet of Chemistry, Peking University, Beijing, 100871
  • Received:1992-01-11 Revised:1992-07-18 Online:1993-01-24 Published:1993-01-24

Abstract: Five chromogenic substrates for chymotrypsin, P3-P2-Pl-p-NA (P1,P2,P3 = Phe, Leu, Ala, Pro, Asn) have been synthesized.The influence of amino acid on the specificity was given by kinetic analysis of the hydrolysis of the peptide by bovine chymotrypsin.Though most of the substrates are suitable to chymotrypsin, Ala-Ala-Phe-p-NAis the best.

Key words: chromogenic substrate, Synthesis, Kinetics, Chymotrypsin

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