Studies on Kinetics of the Competition Between Chelating Agents and Bovine Serum Albumin for Nd(Ⅲ)

Abstract

Abstract: The competition kinetics of six chelating agents(DTPA, EDTA, NTA, Cit, His, Cys) with bovine serum albumin(BSA) for the labeled neodymium (¹⁴⁷Nd(Ⅲ)) was studied by the Centrifugal Ultrafiltration method.The parameter A, which is defined as the dissociation percentage of Nd(I) bound to BSAin the presence of chelating agents, can be used as the criterion for the estimation of the mobilizing ability of chelating agents.Avalues determined experimentally lead to a mobilizing ability order: DTPA>EDTA>NTA>Cit>His>Cys.The experimental results show that the polyaminopolycarboxylated type chelating agent especially DTPAand EDTA, are stronger than the others.The kinetics of these reactions has been studied at pH 6.30 and the mechanism is elucidated.It is found that the reactions of DTPA, EDTAand Cit with BSA·Nd (Ⅲ) follow the association mechanism, but those of NTA, His and Cys, the dissociation mechanism.The kinetic constants were determined respectively.

Key words: Bovine serum albumin, Neodymium, Chela ting competition

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ZHANG Bao-lin, WANG Wen-qing, WU Yong-hui . Studies on Kinetics of the Competition Between Chelating Agents and Bovine Serum Albumin for Nd(Ⅲ)[J]. Chem. J. Chinese Universities, 1992, 13(7): 987.

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Studies on Kinetics of the Competition Between Chelating Agents and Bovine Serum Albumin for Nd(Ⅲ)

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