Precolumn Chelation High Performance Liquid Chromatography Determination of Super- Trace Fe(Ⅱ),Co(Ⅱ ),Fe( Ⅲ)

Abstract

Abstract: This paper describes the separation of Fe( Ⅱ ),Co(Ⅱ ) and Fe( Ⅲ ) chelates with 5-C₁-PADABon C₁₈ bonded stationary phase by RP-Liquid Chro-matography with methanol-water as mobile phase. Heavy-elements such as Fe(Ⅱ), Co(Ⅱ ) and Fe( Ⅲ ) were determined by this chromatographic system. The retention of the chelates depends on central metal ions, mcthanol-water ratio, pHvalue of the mobile plase and concentration of NaH₂PO₄ in mobile phase. The chelates of Fe( Ⅱ ),Co(Ⅱ ),Fe(Ⅲ) at part billion levels were separated within 10 min by using 5μm Nucleosil C₁₈ column(250 × 4mm I.D) with methanol(65)-water(35 containning 2.0g/L NaH₂PO₄) as the mobile phase with pH 2.5. The detection limits of Fe( Ⅱ ), Co( Ⅱ) and Fe( Ⅲ) are 4 × 10^-11g,4 × 10^-11g and 2×10^-11g respectively.

Key words: High performance liquid chromatography, Precolumn chelation, Separation and determination

TrendMD:

Zhao Yi, Fu Chengguang . Precolumn Chelation High Performance Liquid Chromatography Determination of Super- Trace Fe(Ⅱ),Co(Ⅱ ),Fe( Ⅲ)[J]. Chem. J. Chinese Universities, 1989, 10(5): 534.

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Precolumn Chelation High Performance Liquid Chromatography Determination of Super- Trace Fe(Ⅱ),Co(Ⅱ ),Fe( Ⅲ)

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