In situ Biomimetic Remineralization of Enamel-like Hierarchical Structures†

doi:10.7503/cjcu20180171

Abstract

Abstract:

The in situ regeneration of enamel-like hierarchical structures were carried out under biomimetic condition using etched enamel as the template. The morphology, chemical composition, mcriohardness, biocompatibility and mechanical properties of the remineralized layer were investigated by scanning electron microscopy(SEM), energy dispersive spectroscopy(EDS), X-ray diffraction(XRD) and Fourier transform infrared spectroscopy(FTIR). Furthermore, the enamel layers remineralized under biomimetic conditions and hydrothermal condition were compared. The results showed that the structure of them were similar to that of the natural enamel prisms and interprisms, and the crystal composition were both mainly fluorapatite. However, the structures, morphology and microhardness of enamel-like hierarchical enamel layer were better than those of the enamel layer obtained under hydrothermal condition.

Key words: Fluorapatite, Biomimetic, Enamel caries, Hierarchical structure

CLC Number:

O611.4

TrendMD:

CUI Yan, ZHANG Kai, CHEN Yixin, SUN Hongchen, HUANG Yang, WANG Dandan. In situ Biomimetic Remineralization of Enamel-like Hierarchical Structures^†[J]. Chem. J. Chinese Universities, 2018, 39(6): 1121.

Figures/Tables 7

Fig.1 SEM images with different magnifications(A—C) and EDS pattern(D) of remineralized layer under biomimetic condition

Fig.2 SEM images of etched natural enamel with different magnifications

Fig.3 SEM images with different magnifications(A—C) and EDS pattern(D) of remineralized layer under hydrothermal condition

Fig.4 FTIR spectra of the remineralized layer under biomimetic condition(a) and under hydrothermal condition(b)

Fig.5 XRD patterns of the remineralized layer under biomimetic condition(a) and under hydrothermal condition(b)

Fig.6 Surface microhardness(SMH) of the samplesa. Natural enamel; b. etched enamel; c. under biomimetic conditions; d. under hydrothermal conditions.

Fig.7 Effect of different concentration of samples on cell proliferation Natural enamel; Biomimetic conditions; Hydrothermal conditions.

References 21

[1]	Zhou Y. Z., Cao Y., Liu W., Chu C. H., Li Q. L., ACS Appl. Mater. Interfaces, 2012, 4(12), 6901—6910
[2]	Kumar R., Prakash K. H., Cheang P., Gower L., Khor K. A., J. R. Soc. Interface, 2008, 5(21), 427—439
[3]	Baldassarri M., Margolis H. C., Beniash E., J. Dent. Res., 2008, 87(7), 645—649
[4]	Daculsi G., Menanteau J., Kerebel L. M., Mitre D., Calcif. Tissue Int., 1984, 36(5), 550—555
[5]	Cui F. Z., Ge J., J. Tissue Eng. Regen. Med., 2007, 1(3), 185—191
[6]	Du C., Falini G., Fermani S., Abbott C., Moradian-Oldak J., Science, 2005, 307(5714), 1450—1454
[7]	Gibson C. W., Yuan Z. A., Hall B., Longenecher G., Chen E., J. Biol. Chem., 2001, 276, 31871—31875
[8]	Fincham A. G., Moradian-Oldak J., Simmer J. P., J. Struct. Biol., 1999, 126, 270—299
[9]	Cao C. Y., Mei M. L., Li Q., Mater., 2015, 8(6), 2873—2886
[10]	Moradian-Oldak J., Ruan Q., Chitosan-Amelogenin Hydrogel for In situ Enamel Grouth, Patent AP, US2014/0186273 A1, 2014-07-03
[11]	Fang P. A., Conway J. F., Margolis H. C., Simmer J. P., Proc. Natl. Acad. Sci . USA, 2011, 108, 14097—14102
[12]	Yang X., Wang L., Qin Y., Sun Z., Henneman Z. J., J. Phys. Chem., 2010, 114, 2293—2300
[13]	Pan H., Tao J., Xu X., Tang R., Langmuir, 2007, 23(17), 8972—8981
[14]	Paine M. L., White S. N., Luo W., Fong H., Sarikaya M., Matrix Biol., 2001, 20, 273—292
[15]	Xing L., Zhang K., Huang Y., J. Oral Sci., 2013, (4), 301—304
	(邢琳, 张恺, 黄洋. 口腔医学研究, 2013, (4), 301—304)
[16]	Li L., Mao C. Y., Wang J. M., Xu X., Pan H., Adv. Mater., 2011, 23(40), 4695—4701
[17]	Wu D., Yang J., Li J., Chen L., Tang B., Chen X. Y., Wu W., Li J. S., Biomater., 2013, 34(21), 5036—5047
[18]	Yin Y., Yun S., Fang J., Chen H., Chem.Commun., 2009, 63(39), 5892—5894
[19]	Chen H., Tang Z., Liu J., Sun K., Chang S. R., Peters M. C., Mansfield J. F., Czajka-Jakubowska A., Adv. Mater., 2006, 18(14), 1846—1851
[20]	Pan H., Tao J., Yu X., Fu L., Zhang J., J. Phys. Chem. B, 2008, 112(24), 7162—7165
[21]	Featherstone J. D. B., J. Am. Dent. Assoc., 2000, 131(7), 887—889