doi:10.7503/cjcu20170548

Figures/Tables 5

Table 1 Physicochemical properties of transgenic Cry1Ac protein and the reference protein BSA[8,18]

Protein	Cry1Ac				Bovine serum albumin(BSA)
Molecular weight	46990				66400
Molecular shape	Elongated				Heart shaped
Molecular size	7.6 nm×6.2 nm×5.0 nm				Side length 7.5 nm; thickness 5 nm
Conformational stability	High				Low
Isoelectric point	Total	Domain Ⅰ	Domain Ⅱ	Domain Ⅲ	Total	Domain Ⅰ	Domain Ⅱ	Domain Ⅲ
	5.5	4.66	9.34	9.39	4.7	5.5	5.3	7.6

Fig.1 Structure schematic of Cry1AC protein(A) and BSA(B)

Fig.2 Fluorescence of pyrene at 1cmc concentration of surfactants(A) and the intensity of fluorescence(I1) at different surfactant concentrations(B)

Fig.3 Fluorescence behaviors of pyrene with Cry1Ac, BSA and different concentrations of surfactants Note：(A) RL+Cry1AC; (B) RL+BSA; (C) SDS+Cry1AC; (D) SDS+BSA; (E) CTAB+Cry1AC; (F) CTAB+BSA; (G) Tween 80+Cry1AC; (H) Tween 80+BSA.

Fig.4 I1/I3 values of pyrene with different concentrations of surfactants and proteins Note：Surfactant: (A) RL; (B) SDS; (C) CTAB; (D) Tween 80.

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