Preparation and Drug Release of Anti-cancer Fe3O4@ZIF-8@PA System Loaded with Drug†

doi:10.7503/cjcu20170176

Abstract

Abstract:

An anti-cancer and loading drug system was prepared. Fe₃O₄ nanopartiles prepared by hydrothermal method was chosen as substrate to design and fabricate pH responsive magnetic nanocomposites Fe₃O₄@ZIF-8@PA with core-shell structure. This nanomaterial exhibited high saturation of 35.46 A·m²/g and maintained well magnetic property. Fe₃O₄ nanopartiles showed spherical structure and good dispersion. Compared with the substrate, the particle size of composite microsphere increased significantly, but still conform to the ideal carrier material size. In addition, the carriers with porous structures showed large surface area, high drug loading efficiency and capacity for doxorubicin(DOX) of up to 96.4% and 144.6 mg/g. The drug release of nanopartiles loaded with drug was studied in pH=7.4 and 5.0. The total released percentage of two kinds of pH were 39.8% and 78.6%, respectively. The drug releasing experiment was also to perform to verify the constructed nanocarriers excellent pH-response. The introduction of phytic acid with inhibition to cancer cells made it possible that the carriers could kill tumor cells. The methyl thiazolyl tetrazolium(MTT) test act on human osteosarcoma cell line(MG-63) was also carried out in vitro to demonstrate the combination effect of anticancer between the carriers and DOX.

Key words: pH-responsiveness, Combination therapy, Magnetic nanocomposite, Core-shell structure, Phytic acid, Drug release

CLC Number:

O614
O636

TrendMD:

WANG Xiaodan, XU Dandan, LÜ Weizhong, LIU Jingyuan, LIU Qi, JING Xiaoyan, WANG Jun. Preparation and Drug Release of Anti-cancer Fe₃O₄@ZIF-8@PA System Loaded with Drug^†[J]. Chem. J. Chinese Universities, 2017, 38(11): 1927.

Figures/Tables 9

Scheme 1 Schematic representation of basic constitution of Fe3O4@ZIF-8@PA nanoparticles, loading and multi-responsive releasing of DOX and the nanocarriers combining with DOX acting in MG-63 cells

Fig.1 SEM images(A—C)and TEM images(D—F) of Fe3O4(A, D), Fe3O4@ZIF-8(B, E) and Fe3O4@ZIF-8@PA(C, F) nanoparticlesInset of (F) is particle size distribution curve of Fe3O4@ZIF-8@PA.

Fig.2 FTIR spectra of pure ZIF-8(a), Fe3O4@ZIF-8(b), Fe3O4@ZIF-8@PA(c), PA(d) and Fe3O4(e)

Fig.3 XRD patterns of pure ZI F-8(a), Fe3O4@ZIF-8(b), Fe3O4@ZIF-8@PA(c) and Fe3O4(d)

Fig.4 TG curves of ZIF-8(a) and Fe3O4@ZIF-8@PA(b) nanoparticles

Fig.5 Nitrogen adsorption-desorption isotherm with pore size distribution curves in the inset of Fe3O4@ZIF-8(A) and Fe3O4@ZIF-8@PA(B)

Fig.6 Magnetic hysteresis curves of Fe3O4@ZIF-8@PA(a), Fe3O4@ZIF-8(b) and Fe3O4(c)Inset: magnetic separation experiment.

Fig.7 Absorbance spectra of bare Fe3O4@ZIF-8@PA nanocarriers(a), Fe3O4@ZIF-8@PA-DOX(b) and free DOX(c)(A) and release of DOX from Fe3O4@ZIF-8@PA-DOX in buffer solutions at pH=7.4(a) and 5.0(b)(B)

Fig.8 Cell viabilities of pure DOX, Fe3O4@ZIF-8@PA-DOX, Fe3O4@ZIF-8@PA and Fe3O4@ZIF-8 with the same concentration(A) and pure DOX and Fe3O4@ZIF-8@PA-DOX nanoparticles with different concentrations to MG-63 cells examined by MTT assay(B)

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Preparation and Drug Release of Anti-cancer Fe₃O₄@ZIF-8@PA System Loaded with Drug^†

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