Fabrication of Drug Loaded Fluorescent Nanoparticles and Its Biological Application in MCF-7 Breast Cancer Cell

doi:10.7503/cjcu20170120

Chem. J. Chinese Universities ›› 2017, Vol. 38 ›› Issue (5): 860.doi: 10.7503/cjcu20170120

• Polymer Chemistry • Previous Articles Next Articles

Fabrication of Drug Loaded Fluorescent Nanoparticles and Its Biological Application in MCF-7 Breast Cancer Cell

ZHANG Haipeng¹, HAN Bing¹, JIA Zhizhen³, DING Rongbo¹, XU Bin², XU Weiqing², FAN Zhimin¹

1. The First Hospital of Jilin University, Changchun 130021, China;
2. State Key Laboratory for Supramolecular Structure and Materials, Jilin University, Changchun 130012, China;
3. College of Computer Science and Information Technology, Northeast Normal University, Changchun 130117, China

Received:2017-02-27 Revised:2017-04-18 Online:2017-05-10 Published:2017-04-18
Supported by:
Supported by the Natural Science Foundation of Jilin Province Science and Technology Department, China(No.20150101205JC), the Jilin University Bethune Scientific Research Support Plan, China(No.2013205023) and the Youth Foundation of the First Hosptial of Jilin University, China(No.JDYY72016040).

Abstract

Abstract:

Drug-loaded fluorescent nanoparticles(DPBA/PTX@PEG-PDLLA) were prepared by amphiphilic polymers(PEG-PDLLA) coated with AIE dyes(DPBA) and paclitaxel(PTX). The effects of capacity of DPBA and PTX on the photophysical properties of nanoparticles were investigated. In addition, the drug release in vitro of nanoparticles as well as the inhibitory effect of nanoparticles on breast cancer MCF-7 cells were investigated. Moreover, the nanoparticle uptake of MCF-7 cells was observed. As conclusion, the DPBA/PTX@PEG-PDLLA nanoparticles exhibit strong red emission(654 nm) with high fluorescence quantum yield up to 25%. The nanoparticles have uniform size and good biocompatibility. The freshly prepared nanoparticles have pretty drug release ability, and the cumulative release rate of 48 h can reach 25.1%. The results of in vitro MTT and CLSM experiments show that DPBA/PTX@PEG-PDLLA nanoparticles not only have good inhibiting ability with the cell proliferation of MCF-7 tumor cells, but also can be uptake by tumor cell and then absorbed by their cytoplasmic fluorescence imaging.

Key words: Drug loaded fluorescent nanoparticles, Self-assembly, Drug release, Bioimaging, Paclitaxel

CLC Number:

O631
O629

TrendMD:

ZHANG Haipeng, HAN Bing, JIA Zhizhen, DING Rongbo, XU Bin, XU Weiqing, FAN Zhimin. Fabrication of Drug Loaded Fluorescent Nanoparticles and Its Biological Application in MCF-7 Breast Cancer Cell[J]. Chem. J. Chinese Universities, 2017, 38(5): 860.

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Fabrication of Drug Loaded Fluorescent Nanoparticles and Its Biological Application in MCF-7 Breast Cancer Cell

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