UPLC-QTOF-MS-based Metabolomics Study on Ischemic Stroke Patients†

doi:10.7503/cjcu20170153

Abstract

Abstract:

Ultra-performance liquid chromatography with quadrupole-time-of-flight mass spectrometry(UPLC-QTOF-MS) based metabolomics was used to study the metabolism profiling of the plasma from the patients with ischemic stroke, aiming to seek the potential biomarkers of ischemic stroke. 30 patients with ischemic stroke and 17 healthy controls were enrolled. Principal component analysis and orthogonal partial least squares discriminate analysis were used to investigate the metabolic variations between the patients with ischemic stroke and healthy controls. Metabolic pathways were analyzed. Seven biomarkers were obtained. The levels of sphinganine and phytosphingosine were increased, and the levels of glutamine, pyroglutamic acid and 2-ketobutyric acid were decreased. The results of the pathway analysis showed that sphingolipid metabolism and amino acid metabolism were perturbed, and the energy metabolism was also disturbed.

Key words: Metabolomics, Ultra-performance liquid chromatography with quadrupole-time-of-flight mass spectrometry, Ischemic stroke, Plasma

CLC Number:

O657

TrendMD:

HUANG Yu, GU Caiyun, WU Hanzhong, XIA Xiaoshuang, LI Xin. UPLC-QTOF-MS-based Metabolomics Study on Ischemic Stroke Patients^†[J]. Chem. J. Chinese Universities, 2017, 38(10): 1742.

Figures/Tables 10

References 27

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Item	IS^h(n=30)	HCⁱ(n=17)	P value
Sex(M/F)	15/15	7/10	0.779
Age(years)	65.93±8.09	61.65±8.52	0.288
Smoking(none/active/stopped)	9/16/5	6/7/4	0.802
Drinking(none/active/stopped)	20/8/2	9/5/3	0.736
Body mass index/(kg·m^-2)	24.44±2.18	24.52±2.80	0.286
10^-9 White blood cell/L	7.07±2.26	7.66±4.35	0.563
10^-12 Red blood cell/L	4.48±0.72	4.41±0.24	0.847
RDW^a(%)	13.07±0.97	12.60±0.33	0.684
Hemoglobin/(g·L^-1)	138.69±21.65	135.25±8.34	0.758
10^-9 Platelet/L	221.34±55.20	221.20±50.66	0.991
MPV^b/fL	9.56±1.31	8.88±0.61	0.315
Creatinine/(μmol·L^-1)	73.58±21.90	68.27±37.76	0.553
Glycated hemoglobin	6.70±7.67	5.32±0.42	0.080
ALT^c/(U·L^-1)	15.83±8.47	17.88±7.03	0.583
AST^d/(U·L^-1)	17.24±7.51	16.15±4.59	0.735
Cholesterol/(mmol·L^-1)	4.99±0.92	5.12±1.32	0.711
Triglyceride/(mmol·L^-1)	1.68±0.78	1.87±1.63	0.612
HDL^e/(mmol·L^-1)	1.17±0.30	1.35±0.41	0.134
LDL^f/(mmol·L^-1)	3.19±0.66	2.82±0.86	0.161
Hs-CRP^g/(mg·L^-1)	5.98±9.98	8.36±11.48	0.529

Item	IS^h(n=30)	HCⁱ(n=17)	P value
Sex(M/F)	15/15	7/10	0.779
Age(years)	65.93±8.09	61.65±8.52	0.288
Smoking(none/active/stopped)	9/16/5	6/7/4	0.802
Drinking(none/active/stopped)	20/8/2	9/5/3	0.736
Body mass index/(kg·m^-2)	24.44±2.18	24.52±2.80	0.286
10^-9 White blood cell/L	7.07±2.26	7.66±4.35	0.563
10^-12 Red blood cell/L	4.48±0.72	4.41±0.24	0.847
RDW^a(%)	13.07±0.97	12.60±0.33	0.684
Hemoglobin/(g·L^-1)	138.69±21.65	135.25±8.34	0.758
10^-9 Platelet/L	221.34±55.20	221.20±50.66	0.991
MPV^b/fL	9.56±1.31	8.88±0.61	0.315
Creatinine/(μmol·L^-1)	73.58±21.90	68.27±37.76	0.553
Glycated hemoglobin	6.70±7.67	5.32±0.42	0.080
ALT^c/(U·L^-1)	15.83±8.47	17.88±7.03	0.583
AST^d/(U·L^-1)	17.24±7.51	16.15±4.59	0.735
Cholesterol/(mmol·L^-1)	4.99±0.92	5.12±1.32	0.711
Triglyceride/(mmol·L^-1)	1.68±0.78	1.87±1.63	0.612
HDL^e/(mmol·L^-1)	1.17±0.30	1.35±0.41	0.134
LDL^f/(mmol·L^-1)	3.19±0.66	2.82±0.86	0.161
Hs-CRP^g/(mg·L^-1)	5.98±9.98	8.36±11.48	0.529

No.	t_R/min	m/z Obsd. Calcd.		Error, δ	Metabolite	Formula	MS Fragment (ESI+)	Variance	P	Fold change
1	4.59	302.3055	302.3059	-1.32	Sphinganine^a	C₁₈H₃₉NO₂	302.3	↑^**	0.003	1.46
							285.2
							284.1
							244.0
2	0.76	103.0397	103.0395	1.94	2-Ketobutyric acid^a	C₄H₆O₃	103.0	↓^**	0.002	0.64
							85.1
							57.5
3	5.61	259.1908	259.1909	-0.36	Tetradecanedioic acid^b	C₁₄H₂₆O₄	259.2	↑^*	0.044	1.30
							241.2
							197.2
4	3.69	335.2966	335.2950	4.77	Docosatrienoic acid^b	C₂₂H₃₈O₂	335.2	↑^*	0.025	1.58
							274.2
5	0.72	169.0581	169.0589	-4.73	L-Glutamine^b	C₅H₁₀N₂O₃	169.0	↓^**	0.008	0.70
							129.9
							83.9
6	3.69	318.3003	318.3008	-1.57	Phytosphingosine^a	C₁₈H₃₉NO₃	318.3	↑^**	0.001	1.40
							301.2
							300.27
							256.2
7	0.73	130.0505	130.0504	0.77	Pyroglutamic acid^b	C₅H₇NO₃	130.1	↓^*	0.021	0.79
							84.0

No.	t_R/min	m/z Obsd. Calcd.		Error, δ	Metabolite	Formula	MS Fragment (ESI+)	Variance	P	Fold change
1	4.59	302.3055	302.3059	-1.32	Sphinganine^a	C₁₈H₃₉NO₂	302.3	↑^**	0.003	1.46
							285.2
							284.1
							244.0
2	0.76	103.0397	103.0395	1.94	2-Ketobutyric acid^a	C₄H₆O₃	103.0	↓^**	0.002	0.64
							85.1
							57.5
3	5.61	259.1908	259.1909	-0.36	Tetradecanedioic acid^b	C₁₄H₂₆O₄	259.2	↑^*	0.044	1.30
							241.2
							197.2
4	3.69	335.2966	335.2950	4.77	Docosatrienoic acid^b	C₂₂H₃₈O₂	335.2	↑^*	0.025	1.58
							274.2
5	0.72	169.0581	169.0589	-4.73	L-Glutamine^b	C₅H₁₀N₂O₃	169.0	↓^**	0.008	0.70
							129.9
							83.9
6	3.69	318.3003	318.3008	-1.57	Phytosphingosine^a	C₁₈H₃₉NO₃	318.3	↑^**	0.001	1.40
							301.2
							300.27
							256.2
7	0.73	130.0505	130.0504	0.77	Pyroglutamic acid^b	C₅H₇NO₃	130.1	↓^*	0.021	0.79
							84.0