Study on Fresh Breast Tissues by Raman Spectroscopy Based on Robust Statistics†

doi:10.7503/cjcu20140415

Abstract

Abstract:

A portable Raman spectrometer was used for distinguishing the characteristics of normal, malignant and benign fresh breast tissues. Robust statistics method was used to analyze the Raman spectrum data, and a standard Raman spectral atlas of fresh breast tissues was established. Based on the spectral profiles of the Raman spectral standard atlas, the presence of lipids(1078, 1297, 1437, 1653, 1746 cm^-1) is indicated in normal tissue. And proteins(1259, 1530, 1650 cm^-1) are found in benign and malignant tissues. Among normal, benign and malignant tissues, proteins(1340 cm^-1) and β-carotene(1534 cm^-1) are found to be good discrimination parameters, which are hardly achieved by other typical statistical methods. The established fresh breast tissue Raman spectral standard atlas based on robust statistics laid the foundation of benign and malignant breast lesions identify model.

Key words: Raman spectroscopy, Breast cancer, Robust statistics

CLC Number:

O657

TrendMD:

ZHENG Chao, ZHANG Haipeng, HAN Bing, LIANG Lijia, ZOU Yabin, XU Shuping, LIN Heping, XU Weiqing, FAN Zhimin. Study on Fresh Breast Tissues by Raman Spectroscopy Based on Robust Statistics^†[J]. Chem. J. Chinese Universities, 2015, 36(1): 74.

Figures/Tables 4

References 25

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Breast tissue			Assignment
Normal		Benign	Malignant
870	873	873	Hydroxyproline C—C stretch
962	962	965	Cholesterol
1078	1078	1076	C—O stretch(lipids), or O—P—O stretch(nucleic acids)
1155	1179	1179	Lipids
1261	1259	1259	Amide Ⅲ of proteins
1297	1297	1300	CH₂ deformation(lipids)
	1315	1315	CH₃CH₂ twisting mode of collagen
1340			CH₃CH₂ wagging mode of collagen
1362	1362	1364	CH₃CH₂ symmetric deformation of proteins
1385	1383	1383	CH₃ bend
1437	1435	1435	CH₂ deformation(lipids)
1534	1530	1530	C C stretching mode of β-carotene
	1558	1558	Tryptophan
1653	1650	1650	C C of Lipids
		1675	Amide Ⅰ(collagen)
1746	1743	1743	C O of phospholipids

Breast tissue			Assignment
Normal		Benign	Malignant
870	873	873	Hydroxyproline C—C stretch
962	962	965	Cholesterol
1078	1078	1076	C—O stretch(lipids), or O—P—O stretch(nucleic acids)
1155	1179	1179	Lipids
1261	1259	1259	Amide Ⅲ of proteins
1297	1297	1300	CH₂ deformation(lipids)
	1315	1315	CH₃CH₂ twisting mode of collagen
1340			CH₃CH₂ wagging mode of collagen
1362	1362	1364	CH₃CH₂ symmetric deformation of proteins
1385	1383	1383	CH₃ bend
1437	1435	1435	CH₂ deformation(lipids)
1534	1530	1530	C C stretching mode of β-carotene
	1558	1558	Tryptophan
1653	1650	1650	C C of Lipids
		1675	Amide Ⅰ(collagen)
1746	1743	1743	C O of phospholipids