Studies on Fresh Cervical Cancer Tissues by Raman Spectroscopy†

doi:10.7503/cjcu20140401

Abstract

Abstract:

A portable Raman spectrometer was used for distinguishing the characteristics of cervical cancer, cervical intraepithelial neoplasias Ⅲ(CIN Ⅲ) and normal fresh cervical tissues. Based on spectral profiles, the presence of lipids(817, 1127, 1176, 1450, 1769 cm^-1) is indicated in normal tissue, and proteins(755, 1003, 1372, 1542, 1577 cm^-1) are found in diseased tissues. Between CIN Ⅲ and cervical cancer tissues, proteins(853 and 1542 cm^-1) and nucleic acids(1340 cm^-1) are found to be good discrimination parameters. These three kinds of tissues have significant differences on the ability of forming hydrogen bond between dihydrogen phosphate ester groups, the relative content of DNA, the disorder of methylene, the deformation of amide I band CO and the formation of carotenoids. These features show that Raman spectroscopy has good clinical application potential which can be used to detect cervical cancer tissues, explore the relationships and differences between cervical cancer and CIN Ⅲ and diagnose cervical cancer early.

Key words: Raman spectroscopy, Cervical cancer, Cervical intraepithelial neoplasias Ⅲ(CIN Ⅲ), Peak analysis

CLC Number:

O657

TrendMD:

ZHANG Haipeng, ZHENG Chao, LU Lu, ZOU Yabin, WANG Cuihua, XU Shuping, XU Weiqing, FAN Zhimin, LU Laijin, HAN Bing. Studies on Fresh Cervical Cancer Tissues by Raman Spectroscopy^†[J]. Chem. J. Chinese Universities, 2014, 35(9): 1877.

Figures/Tables 4

References 21

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Normal	CINⅢ	Cancer	Assignment
	534	534	S—S Disulfide stretch in proteins
571	571		Tryptophan/cytosine, guanine
640	640	642	C—C Twisting mode of tyrosine
686	689	689	C—S Stretching mode of cystine
	718	721	C—N(membrane phospholipid head)/adenine
755	755	755	Symmetric breathing of tryptophan
786	784	784	DNA: O—P—O stretch, cytosine, uracil, thymine
817	817		Lipids
853	853	853	Ring breathing mode of tyrosine and C—C stretch of proline ring
928	931	928	C—C Skeletal
1005	1003	1003	Symmetric ring breathing mode of phenylalanine
1096	1096	1094	C—C Stretch, O—P—O stretch(nucleic acids)
1121	1121	1121	C—C Stretching mode of lipids
1176	1176	1176	Lipids
1242	1242	1242	Amide Ⅱ: collagen(CH₂wag, C—N stretch)
1271	1271		Amide Ⅲ of proteins
1316	1316		CH₃, CH₂ Twisting mode of collagen/lipids
1341	1341	1345	Polynucleotide chain(DNA-purine bases)
1375	1375	1372	Amide Ⅲ(α-helix) of proteins
1419	1419	1419	CH₂, CH₃ Wagging mode of collagen
1450	1447	1443	CH₂ Deformation(lipids)
	1515		C C Stretch of carotenoid
	1546	1542	Tryptophan
1558			Tryptophan
	1577	1577	Tryptophan protein
1660	1657	1657	AmideⅠ(collagen)
1769	1769	1766	Lipids

Normal	CINⅢ	Cancer	Assignment
	534	534	S—S Disulfide stretch in proteins
571	571		Tryptophan/cytosine, guanine
640	640	642	C—C Twisting mode of tyrosine
686	689	689	C—S Stretching mode of cystine
	718	721	C—N(membrane phospholipid head)/adenine
755	755	755	Symmetric breathing of tryptophan
786	784	784	DNA: O—P—O stretch, cytosine, uracil, thymine
817	817		Lipids
853	853	853	Ring breathing mode of tyrosine and C—C stretch of proline ring
928	931	928	C—C Skeletal
1005	1003	1003	Symmetric ring breathing mode of phenylalanine
1096	1096	1094	C—C Stretch, O—P—O stretch(nucleic acids)
1121	1121	1121	C—C Stretching mode of lipids
1176	1176	1176	Lipids
1242	1242	1242	Amide Ⅱ: collagen(CH₂wag, C—N stretch)
1271	1271		Amide Ⅲ of proteins
1316	1316		CH₃, CH₂ Twisting mode of collagen/lipids
1341	1341	1345	Polynucleotide chain(DNA-purine bases)
1375	1375	1372	Amide Ⅲ(α-helix) of proteins
1419	1419	1419	CH₂, CH₃ Wagging mode of collagen
1450	1447	1443	CH₂ Deformation(lipids)
	1515		C C Stretch of carotenoid
	1546	1542	Tryptophan
1558			Tryptophan
	1577	1577	Tryptophan protein
1660	1657	1657	AmideⅠ(collagen)
1769	1769	1766	Lipids

Raman shift/cm^-1	Normal	CIN Ⅲ	Cervical cancer	P
1345	0.49±0.055	0.55±0.10	0.67±0.086	<0.001
1003	0.10±0.052	0.19±0.053	0.21±0.067	<0.001
1443	0.66±0.17	0.63±0.13	0.62±0.18	0.572

Raman shift/cm^-1	Normal	CIN Ⅲ	Cervical cancer	P
1345	0.49±0.055	0.55±0.10	0.67±0.086	<0.001
1003	0.10±0.052	0.19±0.053	0.21±0.067	<0.001
1443	0.66±0.17	0.63±0.13	0.62±0.18	0.572