Screening DNA Binders from Isoflavone Extracts of Red Clover by Centrifugal Ultrafiltration-LC-MSn

doi:10.7503/cjcu20120467

Chem. J. Chinese Universities ›› 2013, Vol. 34 ›› Issue (2): 331.doi: 10.7503/cjcu20120467

• Analytical Chemistry • Previous Articles Next Articles

Screening DNA Binders from Isoflavone Extracts of Red Clover by Centrifugal Ultrafiltration-LC-MSⁿ

MA Lei^1,2, WANG Zhao-Fu¹, CHEN Li-Na^1,2, SONG Feng-Rui¹, LIU Zhi-Qiang¹, LIU Shu-Ying¹

1. Changchun Center of Mass Spectrometry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China;
2. University of Chinese Academy of Sciences, Beijing 100049, China

Received:2012-05-14 Online:2013-02-10 Published:2013-01-21

Abstract

Abstract:

Centrifugal ultrafiltration and LC-MS methods were combined to screen DNA-binding agents from traditional Chinese medicine red clover isoflavone extracts. The results show that 10 red clover isoflavones have different binding degrees with DNA. The sample was analyzed by LC-MSⁿ for identifying structures. Among these flavones studied, the strongest five potential DNA-binding agents are irilone-4'-O-β-D-glucoside, glycitin, pratensein, irilone and biochanin A, respectively. A rapid centrifugal ultrafiltration-LC-MS platform for screening and identification of DNA binders from the herbal extract and other complex system was established.

Key words: Trifolium pratense L., DNA, Centrifugal ultrafiltration, LC-MS

CLC Number:

O657.63

TrendMD:

MA Lei, WANG Zhao-Fu, CHEN Li-Na, SONG Feng-Rui, LIU Zhi-Qiang, LIU Shu-Ying. Screening DNA Binders from Isoflavone Extracts of Red Clover by Centrifugal Ultrafiltration-LC-MSⁿ[J]. Chem. J. Chinese Universities, 2013, 34(2): 331.

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Screening DNA Binders from Isoflavone Extracts of Red Clover by Centrifugal Ultrafiltration-LC-MSⁿ

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