高等学校化学学报

高等学校化学学报 ›› 1998, Vol. 19 ›› Issue (5): 714.

• 研究简报 • 上一篇    下一篇

吩嗪染料与DNA分子相互作用的紫外-可见光谱研究

曹瑛, 何锡文   

  1. 南开大学化学系, 天津, 300071
  • 收稿日期:1997-05-21 出版日期:1998-05-24 发布日期:1998-05-24
  • 通讯作者: 何锡文
  • 作者简介:曹瑛,女,27岁,博士研究生.
  • 基金资助:

    国家自然科学基金;国家教育委员会博士点专项基金

Studies of Interaction of Phenazine Dye and DNA by UV-Vis Spectrum

CAO Ying, HE Xi-Wen    

  1. Department of Chemistry, Nankai University, Tianjin, 300071
  • Received:1997-05-21 Online:1998-05-24 Published:1998-05-24

PDF (PC)

被引次数

84

关键词: 溶液状态, 嵌插作用, DNA, 吩嗪染料

Abstract: The solution state of Neutral Red(NR) with or without DNAwas investigated by UV-Vis spectrum. NRmolecules are dimerized in water. The NRabsorbance spectra of monomer and dimer are obtained by calculating and the calculated dimerization equilibrium constant Kis 8.4×104>/sup> L·mol-1 . NR and DNA strongly interact with each other. The apparent binding constant(Kap) and the binding numbers of NRand DNAfrom absorption spectra data are 5.79×104 L·mol-1 and 43, respectively. Both the influence of ionic strength on the interaction between N Rand DNA and NR on Tm of DNAare also studied.

Key words: Solution state, Intercalation, DNA, Phenazine dye

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