高等学校化学学报

高等学校化学学报

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毛细管电泳间接电致化学发光灵敏检测去甲肾上腺素及其在尿样分析中的应用

刘彦明, 曹俊涛, 郑艳丽   

  1. 信阳师范学院化学化工学院, 信阳 464000
  • 收稿日期:2007-10-08 修回日期:1900-01-01 出版日期:2008-01-10 发布日期:2008-01-10
  • 通讯作者: 刘彦明

Sensitive Determination of Norepinephrine by Capillary Electrophoresis with Indirect Electrochemiluminescence Detection and Its Application to Analysis of Human Urine

LIU Yan-Ming*, CAO Jun-Tao, ZHENG Yan-Li   

  1. College of Chemistry and Chemical Engineering, Xinyang Normal University, Xinyang 464000, China
  • Received:2007-10-08 Revised:1900-01-01 Online:2008-01-10 Published:2008-01-10
  • Contact: LIU Yan-Ming

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被引次数

25

摘要： 基于3种肾上腺素对三联吡啶钌-三丙胺发光体系具有强烈抑制的特性, 建立了一种毛细管电泳分离-间接电致化学发光(CE-IECL)灵敏检测NE、脱氧肾上腺素和异丙肾上腺素的新方法, 并将该方法成功地应用于人尿样分析. 结果表明， 糖尿病肾病患者尿样中的NE含量显著比健康人的高.

关键词: 毛细管电泳, 间接电致化学发光检测, 糖尿病肾病患者, 去甲肾上腺素, 尿样分析

Abstract: A new method for sensitive and rapid indirect electrochemiluminescence(IECL) detection of norepinephrine(NE), synephrine, and isoproterenol with capillary electrophoresis(CE) was developed. IECL detection is based on their quenching effects on Ru(bpy)32+/tripropylamine(TPA) system. Under the optimum conditions, the detection limits(S/N=3) were 2.6×10-8 mol/L for NE, 6.6×10-9 mol/L for synephrine, and 8.4×10-8 mol/L for isoproterenol. The RSD of migration time and peak area were less than 1.3% and 4.4%, respectively. The proposed method was successfully applied to the analysis of NE in the urine samples of 10 healthy people and 10 diabetic nephropathy patients. The results indicate that the content of NE in diabetic nephropathy patients is much higher than that in healthy people.

Key words: Capillary electrophoresis, Indirect electrochemiluminescence detection, Diabetic nephropathy patient, Norepinephrine, Urine sample analysis

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