高等学校化学学报

高等学校化学学报

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双重对象同时检测的新型荧光纳米粒子传感器的制备

费砚群1, 罗贵民1, 冯国栋2, 费强2, 陈焕文2, 梁学凯2, 赵晓君3, 郇延富1,2   

    1. 吉林大学分子酶学工程教育部重点实验室, 长春 130021;
    2. 吉林大学化学学院, 长春 130012;
    3. 北达科他大学化学系, 大福克斯 58203
  • 收稿日期:2007-09-21 修回日期:1900-01-01 出版日期:2008-01-10 发布日期:2008-01-10
  • 通讯作者: 郇延富,赵晓君

Synthesis of Fluorescent Nanoparticle Sensors for Simultaneous Determination of Double Targets

FEI Yan-Qun1, LUO Gui-Min1, FENG Guo-Dong2, FEI Qiang2, CHEN Huan-Wen2, LIANG Xue-Kai2, ZHAO Xiao-Jun3*, HUAN Yan-Fu1,2*   

    1. Key Laboratory for Molecular Enzymology & Engineering, Ministry of Education, Jilin University, Changchun 130021, China;
    2. College of Chemistry, Jilin University, Changchun 130012, China;
    3. Department of Chemistry, University of North Dakota, Grand Forks 58203, USA
  • Received:2007-09-21 Revised:1900-01-01 Online:2008-01-10 Published:2008-01-10
  • Contact: HUAN Yan-Fu,ZHAO Xiao-Jun

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被引次数

9

摘要： 报道了一种基于荧光共振能量转移理论合成的新型纳米粒子. 新型纳米粒子A中掺杂了由亲和素-生物素桥联并可发生荧光共振能量转移(FRET)的供体染料和受体染料; 新型纳米粒子B中只掺杂了供体染料. 在使用供体染料的特征激发波长同时激发纳米粒子A和B时, 纳米粒子A由于荧光共振能量转移而发射出受体染料的特征波长, 而纳米粒子B则只发射供体染料的特征波长. 这样, 在单一波长激发下, 可很容易地实现双重对象同时检测.

关键词: 纳米粒子, 双重对象, 同时检测, 荧光共振能量转移

Abstract: New fluorescent silica nanoparticles for sensors simultaneous determination of double targets were synthesized via a reverse microemulsion method based on the fluorescence resonance energy transfer(FRET). Nanoparticle A was doped with a dye complex which consisted of two dye molecules, fluorescein and tetramethylrhodamine. These dye molecules were first linked with avidin and biotin, respectively. They then formed a stable dye complex through avidin-biotin bridge within the distance of FRET. Nanoparticle B was doped with fluorescein only. As a result of this combination, the nanoparticles emit double wavelengths when a single excitation wavelength is used. The results show that the fluorescent nanoparticles are promising fluorescent labeling reagents for the sensitive detection of double targets.

Key words: Nanoparticle, Double targets, Simultaneous determination, Fluorescence resonance energy transfer

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