高等学校化学学报

高等学校化学学报 ›› 2000, Vol. 21 ›› Issue (5): 710.

• 研究简报 • 上一篇    下一篇

PAP-H2O2-HRP酶促反应产物光谱及电化学研究

张书圣1, 焦奎1, 李惠静1, 陈洪渊2   

  1. 1. 青岛化工学院应用化学系, 青岛 266042;
    2. 南京大学化学系, 南京 210093
  • 收稿日期:1999-06-30 出版日期:2000-05-24 发布日期:2000-05-24
  • 通讯作者: 焦奎(1945年出生),男,博士,教授,博士生导师,主要从事电分析化学研究·
  • 基金资助:

    国家自然科学基金(批准号:29775012);长春应用化学研究所电分析化学开放实验室基金

Spectroscopic and Electrochemical Studies on the Enzyme-catalyzed Reaction of PAP-H2O2-HRP

ZHANG Shu-Sheng1, JIAO Kui1, LI Hui-Jing1, CHEN Hong-Yuan2   

  1. 1. Department of Applied Chemistry, Qingdao Institute of Chemical Technology, Qingdao 266042, China;
    2. Department of Chemistry, Nanjing University, Nanjing 210093, China
  • Received:1999-06-30 Online:2000-05-24 Published:2000-05-24

PDF (PC)

关键词: 对氨基酚, 辣根过氧化物酶, 伏安法, 酶催化

Abstract: The pure product of p-aminophenol(PAP)-H2O2-horse peroxidase(HRP) of H2O2 oxidizing PAPcatalyzed by HRPwas prepared with chemical method. The enzyme-catalyzed reaction was investigated by electroanalytical chemistry, UV-Vis, IR,13C NMR, 1H NMR, mass spectrum and elemental analysis etc.. Under the selected enzyme-catalyzed reaction conditions, the oxidation product of PAPwith H2O2 catalyzed by HRPis 3,4-di-[(4-hydroxyphenyl)amino]-6-[(4-hydroxyphenyl)imino]-2,4-cyclo-hexadiene-1-one.

Key words: p-Aminophenol, Horseradish peroxidase(HRP), Voltammetry, Enzyme-catalysis

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