高等学校化学学报

高等学校化学学报 ›› 2000, Vol. 21 ›› Issue (3): 358.

• 论文 • 上一篇    下一篇

多元线性模型计算基于荧光猝灭原理的膜传感器对分析物的响应参数

李伟1,2,3, 陈坚2, 相秉仁1, 安登魁1   

  1. 1. 中国药科大学分析计算中心, 南京 210009;
    2. 新疆医科大学药学院, 乌鲁木齐 830054;
    3. 厦门大学化学化工学院现代分析科学教育部重点实验室, 厦门 361005
  • 收稿日期:1999-05-19 出版日期:2000-03-24 发布日期:2000-03-24
  • 通讯作者: 李　伟(1962年出生),男,博士,副教授,现在厦门大学化学系做博士后工作,从事荧光光谱分析研究.
  • 基金资助:

    国家自然科学基金(批准号:29775022)

A Multivariate Linear Model for Calculating Response Feature Parameters of Chemical Sensor Based on Fluorescence Quenching for Analytes

LI Wei1,2,3, CHEN Jian2, XIANG Bing-Ren1, AN Deng-Kui1   

  1. 1. Research Center for Computer Science and Analytical Chemistry, China Pharmaceutical University, Nanjing 210009, China;
    2. School of Pharmaceutical Science, Xinjiang Medical University, Urumqi 830054, China;
    3. The Key Laboratory of Analytical Sciences of MOE and Department of Chemistry, Xiamen University, Xiamen 361005, China
  • Received:1999-05-19 Online:2000-03-24 Published:2000-03-24

PDF (PC)

被引次数

9

摘要： 根据基于荧光猝灭原理的化学传感膜的结构特征和发光机理,推导并提出量化作用于膜传感器的多种荧光猝灭因素的多元线性模型.结合三杯法,该模型可简便地求出反映不同荧光猝灭机理的特征参数.通过建立的数学模型,测定并计算了芘丁酸膜传感器对应于呋喃妥因等23种药物多种荧光猝灭因素的响应数据和响应参数.结果表明,传感膜对分析物的响应特征往往能得到主要猝灭因素响应模型的近似反映,这为基于荧光猝灭原理的化学传感器定量分析模型的建立提供了可借鉴的思路和方法.

关键词: 化学传感器, 分子探针, 荧光猝灭, 多元线性模型

Abstract: Amathematical model was deduced and described for the different mechanism of fluorescence quenching to analytes based on the essential quenching models including the dynamic quenching, the resonance energy transfer and the inner filter effects involving absorption of both excited light from the light source and emitted light from membrane. The model reported here indicated that the logarithmic functions of the intensity of fluorescence modulation in virtue of multiple quenching factors conform to the rule of addition. The response feature parameters of chemical sensor based on fluorescence quenching for analyses could be calculated by combining the multivariate linear model with a tri cup method. Aconception based on regarding the chemical sensor as the second source of light was advanced to get more useful information on analytes. The pyrenebutyric acid was chosen as a fluoroprobe for constructing the chemical sensor to response a series of the therapeutic drugs. Atrain of thought for constructing the quantitative analysis model for the chemical membrane sensors based on the fluorescence quenching was afforded in the light of the experimental results.

Key words: Chemical sensors, Molecule probe, Fluorescence quenching, Multivariate linear model

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