高等学校化学学报

高等学校化学学报 ›› 2000, Vol. 21 ›› Issue (7): 995.

• 论文 •    下一篇

Co(phen)2TATP3+与DNA在旋转金盘金环电极上的相互作用研究

李红1, 蒋雄2, 王雷3, 叶保辉1, 计亮年1   

  1. 1. 中山大学化学与化工学院, 广州 510275;
    2. 华南师范大学化学系, 广州 510631;
    3. 广州师范学院化学系, 广州 510405
  • 收稿日期:1999-10-20 出版日期:2000-07-24 发布日期:2000-07-24
  • 通讯作者: 计亮年(1934年出生),男,教授,博士生导师,主要从事生物无机化学研究.
  • 基金资助:

    国家自然科学基金(批准号:29871036);广东省自然科学基金(批准号:970211)资助

Electrochemical Studies on the Interactions of the Complex of Co(phen)2TATP3+ with DNA at Rotating Au Au Electrode

LI Hong1, JIANG Xiong2, WANG Lei3, YE Bao-Hui1, JI Liang-Nian1   

  1. 1. School of Chemistry and Engineering, Zhongshan University, Guangzhou 510275, China;
    2. Department of Chemistry, South China Normal University, Guangzhou 510631, China;
    3. Department of Chemistry, Guangzhou Normal College, Guangzhou 510405, China
  • Received:1999-10-20 Online:2000-07-24 Published:2000-07-24

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摘要： 在pH=7.2的Tris缓冲溶液中,利用旋转环盘电极法研究了金电极上Co(phen)2TATP3+与DNA的相互作用,并根据扩散控制和电化学控制下得到的各种参数,对它们作用的模式进行了讨论.发现当一定量的DNA存在时,Co(phen)2TATP3+的扩散系数、还原反应的传递系数和半波电位下的速率常数、还原产物Co(phen)2TATP2+的收集系数和脱出率等都发生了较大幅度的减小.利用Co(bpy)33+进行比较研究表明,Co(phen)2TATP3+与Co(bpy)33+在加入DNA后在收集系数和传递系数变化上存在较大的差异.

关键词: 多吡啶钴(Ⅲ)配合物, DNA, 金电极, 旋转环盘电极, 插入作用

Abstract: The interaction between complex Co(phen)2TATP 3+ (phen=1,10 phenanthroline, TATP= 1,4,8,9-tetranitrogen-trisphene) and DNAhas been investigated by using rotating ring-disk electrode technique, and its models are discussed from the parameters obtained under diffusion and electrochemical control, respectively. It has been found that the values of the following electrochemical parameters: the apparent diffusion coefficient, transfer coefficient, rate constant at half-wave potential, collection coefficient, desorption efficiency etc., for the complex of Co(phen)2TATP 3+ decrease significantly in the presence of DNAcompared with that in the absence of DNA. It is shown from the comparative studies of Co(bpy)33+ that, in the presence of DNA, significant differences exist in the change of transfer coefficient and collection coefficient between Co(bpy)33+ and Co(phen)2TATP 3+ .

Key words: Polypyridyl cobalt(Ⅲ) complex, DNA, Gold electrode, Rotating ring disk electrode, Intercatative interaction

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