乌龙茶多糖OTPS 2-1的光谱特性、形貌特征及热特性研究

高等学校化学学报 ›› 2004, Vol. 25 ›› Issue (12): 2263.

乌龙茶多糖OTPS 2-1的光谱特性、形貌特征及热特性研究

倪德江, 陈玉琼, 谢笔钧, 张芸, 周继荣

华中农业大学食品科技学院, 武汉430070

收稿日期:2003-10-27 出版日期:2004-12-24 发布日期:2004-12-24
基金资助:
国家自然科学基金(批准号:30270939);湖北省自然科学基金(批准号:2002AB101);中国科学院上海有机化学研究所生命有机国家重点实验室资助

Spectrum, Morphological and Thermal Characteristics of OTPS 2-1 in Polysaccharides from Oolong Tea

NI De-Jiang, CHEN Yu-Qiong, XIE Bi-Jun, ZHANG Yun, ZHOU Ji-Rong

College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China

Received:2003-10-27 Online:2004-12-24 Published:2004-12-24

摘要/Abstract

摘要： 采用纤维素和葡聚糖凝胶柱层析法分离纯化,获得乌龙茶多糖主要组分OTPS2-1.经GC,HPLC,UV,IR和GPC-LLS证实OTPS2-1是富含糖醛酸和少量蛋白质的三元糖复合物,相对重均分子量为8.877×10⁴,糖基由半乳糖、葡萄糖、阿拉伯糖、岩藻糖和鼠李糖组成,其摩尔比为7.58:2.14:7.05:1.76:1.02.利用AFM,QELS及LSCM观察OTPS2-1在溶液中的形貌特征,结果表明,OTSP2-1在溶液中呈直径0.2～0.5μm、高度0.3～0.6μm的短棒形不均一聚集体,并具有强烈的荧光,溶液的pH值显著影响其聚集行为.pH值、离子强度和络合物等环境因素的改变,均导致多糖立体构象和非对称性的变化.OTPS2-1在SEM低倍镜下呈折叠卷曲形貌,在高倍镜下呈现平整的图像.综合热分析结果表明,在加热过程中,多糖有3次大的质量损失过程,出现3次吸热峰和放热峰.提示茶叶经过半发酵处理后,多糖组成、分子质量分布以及分子间相互作用可引起微观形貌、链构象和聚集行为的变化,有利于提高生物活性.

关键词: 乌龙茶多糖, 光谱, 形貌, 热分析

Abstract: OTPS 2-1, a main constituent of OTPS, was obtained by DEAE-52 and Sephadex G-150 column chromatography. It was found that OTPS 2-1 was a trinal compound of polysaccharide containing rich uronic acid and little protein by using methods of UV, IR, GC, HPLC and GPC-LLS. The results showed that the average molecular weight of OTPS 2-1 was 8.877×10⁴, and it was composed of Gal, Glu, Ara, Fuc and Rha with a molar ratio of 7.58:2.14:7.05:1.76:1.02. By using the technique of AMF, QELS, CD and LSCM, the three-dimensional conformation and solution behavior were studied. The results also showed that the molecule of OTPS 2-1 twisted each other, which formed short stick of 0.2-0.5 μm in diameter and 0.3-0.6 μm in length with a strong fluorescent light. The conformation could be changed by the factors of pH, ion intensity and complex compound. By using SEM, the folding and curling image under low-powered microscope, but smooth figure under high-powered microscope were also observed. Thermal analysis of OTPS 2-1 also indicated that there appeared 3 endothermic peaks and 3 exothermic peaks during heating.

Key words: Oolong tea polysaccharides, Spectrum, Morphology, Thermal analysis

中图分类号:

O662

TrendMD:

倪德江, 陈玉琼, 谢笔钧, 张芸, 周继荣. 乌龙茶多糖OTPS 2-1的光谱特性、形貌特征及热特性研究. 高等学校化学学报, 2004, 25(12): 2263.

NI De-Jiang, CHEN Yu-Qiong, XIE Bi-Jun, ZHANG Yun, ZHOU Ji-Rong . Spectrum, Morphological and Thermal Characteristics of OTPS 2-1 in Polysaccharides from Oolong Tea. Chem. J. Chinese Universities, 2004, 25(12): 2263.

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