碱性品红修饰磁性纳米粒子的制备及在分枝杆菌分离中的应用

doi:10.7503/cjcu20120906

高等学校化学学报

碱性品红修饰磁性纳米粒子的制备及在分枝杆菌分离中的应用

察冬梅¹, 韩朝晖¹, 马甜¹, 李步海¹, 刘国诠², 祝伟³

1. 中南民族大学化学与材料科学学院分析化学国家民委重点实验室, 武汉 430074;
2. 中国科学院化学研究所, 北京 100190;
3. 中南民族大学生命科学学院, 武汉 430074

收稿日期:2012-10-05 出版日期:2013-04-10 发布日期:2013-03-21
通讯作者: 李步海,男,教授,博士生导师,主要从事生物和环境分析中富集与分离技术研究.E-mail:libh@scuec.edu.cn E-mail:libh@scuec.edu.cn
基金资助:
湖北省教育厅指导性科研计划项目(批准号:B20110809)和中南民族大学学术团队科研基金项目(批准号:XTZ09005)资助.

Preparation of Basic Fuchsin Modified Magnetic Microspheres and Its Application in Isolation of Mycobacterium

CHA Dong-Mei¹, HAN Zhao-Hui¹, MA Tian¹, LI Bu-Hai¹, LIU Guo-Quan², ZHU Wei³

1. Key Laboratory for Analytical Chemistry of the State Ethnic Affairs Commission, College of Chemistry and Material Science, South-Central University for Nationalities, Wuhan 430074, China;
2. Institute of Chemistry, The Chinese Academy of Sciences, Beijing 100190, China;
3. College of Life Science, South-Central University for Nationalities, Wuhan 430074, China

Received:2012-10-05 Online:2013-04-10 Published:2013-03-21

摘要/Abstract

关键词: 磁性微球, 碱性品红, 细菌分离, 分枝杆菌

Abstract:

Two kinds of methods were used to prepare basic fuchsin(BF) modified magnetic nanoparticles. The obtained BF modified nanoparticles(MNP-1 and MNP-2) were characterized using transmission electron microscope(TEM) and Fourier transformation infrared(FTIR) spectroscopy before used to adsorb mycobacterium from Staphylococcus aureus and Escherichia coli(E. coli). The effects of pH, adsorption time, concentration of bacteria, temperature, ionic strength and adsorbent dosage on the adsorption of bacterium were studied. The results show that both MNP-1 and MNP-2 have good selectivity for mycobacterium, and can be easily separated by external magnetic field. Under the optimized conditions, the highest adsorption rate for mycobacteria were 65%(MNP-1) and 85%(MNP-2), while only 20% of Staphylococcus aureus and 10% of E. coli can be adsorbed under the same conditions. The adsorption capacity of MNP-1 and MNP-2 for mycobacteria was 1.48?0¹⁰ and 3.25?0¹⁰ cfu/g, respectively.

Key words: Magnetic microspheres, Basic fuchsin, Bacterium separation, Mycobacterium

中图分类号:

O652.6

TrendMD:

察冬梅, 韩朝晖, 马甜, 李步海, 刘国诠, 祝伟. 碱性品红修饰磁性纳米粒子的制备及在分枝杆菌分离中的应用. 高等学校化学学报, doi: 10.7503/cjcu20120906.

CHA Dong-Mei, HAN Zhao-Hui, MA Tian, LI Bu-Hai, LIU Guo-Quan, ZHU Wei. Preparation of Basic Fuchsin Modified Magnetic Microspheres and Its Application in Isolation of Mycobacterium. Chem. J. Chinese Universities, doi: 10.7503/cjcu20120906.

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碱性品红修饰磁性纳米粒子的制备及在分枝杆菌分离中的应用

Preparation of Basic Fuchsin Modified Magnetic Microspheres and Its Application in Isolation of Mycobacterium

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