高等学校化学学报 ›› 2012, Vol. 33 ›› Issue (07): 1462-1465.doi: 10.3969/j.issn.0251-0790.2012.07.017

• 有机化学 • 上一篇    下一篇

4,4'-二(对氨基苯乙炔基)-6,6'-二[N,N-二(乙氧基羰甲基)氨甲基]-2,2'-联吡啶的合成与表征

曹熙杰1, 谢文兵2, 潘利华2, 常宇2, 崔善子1, 张洪杰2, 党丹丹2   

  1. 1. 长春工业大学化学工程学院, 长春 130012;
    2. 中国科学院长春应用化学研究所, 长春 130022
  • 收稿日期:2011-12-15 出版日期:2012-07-10 发布日期:2012-07-10
  • 通讯作者: 潘利华, 女, 研究员, 主要从事时间分辨荧光免疫分析研究. E-mail: lihuap@ciac.jl.cn E-mail:lihuap@ciac.jl.cn
  • 基金资助:

    国家自然科学基金(批准号: 30070714)资助.

Synthesis and Characterization of 4,4'-Di(p-aminophenylethynyl)-6,6'-bis[N,N-bis(ethoxycarbonylmethyl)amino methyl]-2,2'-bipyridine

CAO Xi-Jie1, XIE Wen-Bing2, PAN Li-Hua2, CHANG Yu2, CUI Shan-Zi1, ZHANG Hong-Jie2, DANG Dan-Dan2   

  1. 1. School of Chemical Engineering, Changchun University of Technology, Changchun 130012, China;
    2. Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China
  • Received:2011-12-15 Online:2012-07-10 Published:2012-07-10

摘要: 以4,4'-二硝基-2,2'-联吡啶-6,6'-二亚甲基双三氟乙酸酯作为起始原料, 经水解、 溴化、 酯化和对氨基苯乙炔取代4步反应合成了4,4'-二(对氨基苯乙炔基)-6,6'-二[N,N-二(乙氧基羰甲基)氨甲基]-2,2'-联吡啶. 通过红外光谱、 核磁共振波谱、 高分辨质谱等表征了该化合物的结构. 该化合物经水解后与铕离子形成稀土荧光螯合物, 在紫外光激发下, 发射出具有铕离子特征的荧光光谱.

关键词: 固相时间分辨荧光免疫分析, 螯合剂, 中间体, 4,4'-二(对氨基苯乙炔基)-6,6'-二[N,N-二(乙氧基羰甲基)氨甲基]-2,2'-联吡啶

Abstract: In order to obtain a new solid phase time-resolved fluorescence immunoassay chelate, a new intermediate compound 4,4'-(p-aminophenylethynyl)-6,6'-bis[N,N-bis(ethoxycarbonylmethyl)amino methyl]-2,2'-bipyridine was prepared from 4,4'-dinitro-2,2'-bipyridine-6,6'-dimethyl bis(trifluoroacetate) by hydrolyzation, bromination, esterification and substitution. The structure of the compound was charactered by DSC, IR, 1H NMR and HRMS. The compound was hydrolyzed in the solution of europium ion, forming a rare earth fluorescence chelate. The fluorescence spectrum of the chelate excited by an light source has peaks peculiar to the europium ion.

Key words: Solid phase time-resolved fluorescence immunoassay, Chelant, Intermediate, 4,4'-Di(p-aminophenylethynyl)-6,6'-bis[N,N-bis(ethoxycarbonylmethyl)amino methyl]-2,2'-bipyridine

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