高等学校化学学报 ›› 2003, Vol. 24 ›› Issue (6): 1013.

• 研究简报 • 上一篇    下一篇

早期生物矿化过程中紫外吸收动力学曲线上的负峰现象

黄兆龙1,2, 张伟1, 崔福斋1, 蔡强1   

  1. 1. 清华大学材料科学与工程系, 北京 100084;
    2. 蒙自师范高等专科学校化学系, 蒙自 661100
  • 收稿日期:2002-06-26 出版日期:2003-06-24 发布日期:2003-06-24
  • 通讯作者: 崔福斋(1945年出生),男,教授,博士生导师,从事仿生材料和人体医学材料研究.E-mail:cuifz@mail.tsinghua.edu.cn E-mail:cuifz@mail.tsinghua.edu.cn
  • 基金资助:

    国家自然科学基金重点项目(批准号:20031010)资助

A Phenomenon of Negative Peak Appeared in Kinetic Ultraviolet Absorb Curve of Initial Biomineralization

HUANG Zhao-Long1,2, ZHANG Wei1, CUI Fu-Zhai1, CAI Qiang1   

  1. 1. Department of Materials Science and Engineering, Tsinghua University, Beijing 100084, China;
    2. Department of Chemistry, Mengzi Teachers' College, Mengzi 661100, China
  • Received:2002-06-26 Online:2003-06-24 Published:2003-06-24

关键词: 生物矿化, 紫外分光光度法, 胶原, 磷酸钙

Abstract: The ultraviolet photometry is an effective method to investigate the kinetic process of biomineralization because it can be used to monitor the reaction in real time without disturbance. This paper reports an interesting phenomenon in experiment on collagen mineralization. An abnormal negative peak was found in kinetic ultraviolet absorb curve of collagen/calcium phosphate biomineralization system in Tris-HCl buffer(pH=7.4). Influence of several experiment conditions on the negative peak was studied. SEM observation of the sample before and after the negative peak revealed that the crystals morphology and the aggregation of the mineralized collagen fibers are different. It indicated that the negative peak in the kinetic curve is an optical phenomenon related to the compositional and structural change of the mineral, which are the important elements in biomineralization mechanism study.

Key words: Biomineralization, Ultraviolet spectrophotometer, Collagen, Calcium phosphate

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