高等学校化学学报 ›› 2012, Vol. 33 ›› Issue (03): 447-452.doi: 10.3969/j.issn.0251-0790.2012.03.003

• 研究论文: 无机化学 • 上一篇    下一篇

磷酸钙陶瓷颗粒微纳米结构对蛋白吸附的影响

李波1,2,3, 张慧杰3, 廖晓玲1,2, 朱向东3, 范红松3, 张兴栋3   

  1. 1. 重庆科技学院生物材料与活细胞影像技术研究所,重庆 401331;
    2. 重庆科技学院冶金与材料工程学院, 重庆 401331;
    3. 四川大学国家生物医学材料工程技术研究中心, 成都 610064
  • 收稿日期:2011-07-05 出版日期:2012-03-10 发布日期:2012-03-10
  • 通讯作者: 李 波, 男, 博士, 讲师, 主要从事纳米生物材料研究. E-mail:leewave@126.com
  • 基金资助:

    国家自然科学基金青年基金(批准号: 50802060, 81101154), 重庆市科委自然科学基金计划项目(批准号: CSTC, 2010BB5294)和重庆科技学院校内科研基金(批准号: CK2010B27)资助.

Effects of Microstructure of Calcium Phosphate Ceramics Particles on Protein Adsorption

LI Bo1,2,3, ZHANG Hui-Jie3, LIAO Xiao-Ling1,2, ZHU Xiang-Dong3, FAN Hong-Song3, ZHANG Xing-Dong3   

  1. 1. Institute of Biomaterials and Living Cell Imaging Technology,Chongqing University of Science and Technology, Chongqing 401331, China;
    2. School of Metallurgy and Materials Engineering, Chongqing University of Science and Technology, Chongqing 401331, China;
    3. National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, China
  • Received:2011-07-05 Online:2012-03-10 Published:2012-03-10

摘要:

用微波烧结和常规烧结方法分别制备了4种具有纳米和微米结构的磷酸钙陶瓷, 对陶瓷的相组成、 微观结构、 粒度分布、 比表面积、 孔径分布和表面Zeta电位进行了对比分析, 并进一步采用凝胶电泳法考察了陶瓷对牛血清白蛋白/溶菌酶双蛋白的吸附行为. 结果显示, 纳米陶瓷和常规陶瓷具有相似的相组成、 颗粒分布和表面Zeta电位, 但微孔结构、 比表面积和蛋白吸附差异明显. 纳米陶瓷具有较小的晶粒尺寸和更丰富的介孔结构, 使其能吸附更多的牛血清白蛋白和溶菌酶, 表明其具有更强的生物活性.

关键词: 磷酸钙陶瓷, 微纳米结构, 微波, 蛋白吸附

Abstract:

To investigate the effects of physical and chemical properties of nano calcium phosphate ceramics on protein adsorption, two types of nano calcium phosphate particles and two kinds of micron ones were prepared with microwave sintering method and conventional method, respectively. The phase compositions, surface morphologies, particle size distribution, specific surface area, pore size distribution and zeta potentials of four particles were analyzed with X-ray diffraction, scanning electron microscope, dynamic laser system, BET method and zetasizer. Bovine serum album(BSA) and lysozyme(LSZ) adsorbed on ceramics were further investigated by polyacrylamide gel electrophoresis method. The results show that nano and conventional ceramics have similar phase compositions, particle size distribution and zeta potentials. Owing to smaller grain size and more abundant mesoporous structure, nano ceramics adsorb more BSA and LSZ than conventional ones.

Key words: Calcium phosphate ceramics, Micro/nano-structure, Microwave, Protein adsorption

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