载肾上腺髓质素微球复合PLGA/纳米羟基磷灰石支架材料的制备及其生物学活性评价

高等学校化学学报 ›› 2011, Vol. 32 ›› Issue (7): 1622.

载肾上腺髓质素微球复合PLGA/纳米羟基磷灰石支架材料的制备及其生物学活性评价

王林¹,李春艳¹,何盼²,付丽¹,周延民¹,陈学思²

1. 吉林大学口腔医院种植中心, 长春 130021;
2. 中国科学院长春应用化学研究所, 高分子物理和化学国家重点实验室, 长春 130022

收稿日期:2010-11-11 修回日期:2011-03-04 出版日期:2011-07-10 发布日期:2011-06-02
通讯作者: 周延民 E-mail:jlcyspring@126.com
基金资助:
口腔疾病研究国家重点实验室创新人才培养课题基金(批准号: SKLODd2009001)及吉林省产业技术研究与开发项目(批准号: 20101935)资助.

Preparation and Bioactivities of PLGA/Nano-hydroxyapatite Scaffold Containing Chitosan Microspheres for Controlled Delivery of Mutifuncational Peptide-adrenomedullin

WANG Lin¹, LI Chun-Yan¹, HE Pan², FU Li¹, ZHOU Yan-Min^1*, CHEN Xue-Si²

1. Implant Center, Stomatological Hospital of Jilin University, Changchun 130021, China;
2. State Key Laboratory of Polymer Physics and Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, China

Received:2010-11-11 Revised:2011-03-04 Online:2011-07-10 Published:2011-06-02
Contact: Yan-Min ZHOU E-mail:jlcyspring@126.com
Supported by:
口腔疾病研究国家重点实验室创新人才培养课题基金(批准号: SKLODd2009001)及吉林省产业技术研究与开发项目(批准号: 20101935)资助.

摘要/Abstract

摘要： 利用离子乳化交联法制备载肾上腺髓质素的壳聚糖微球，应用热致相分离法制备PLGA/nHA支架材料并将载药微球包裹其中。通过材料的扫描电镜表征，体外释放行为，材料溶血行为，ALP活性的测定，MTT等检测手段综合评价载药支架材料的基本性能及生物学活性。结果表明，微球直径均匀，载药支架孔径大小合适并相互穿通。支架材料的溶血率小于5%，符合医用材料的溶血实验要求。载药支架材料以及支架材料本身对成骨细胞以及血管内皮细胞的增殖以及成骨细胞的分化均有一定的促进作用。

关键词: 壳聚糖, 微球, 聚丙交酯-乙交酯( PLGA), 纳米羟基磷灰石, 肾上腺素质素

Abstract: In the presence of tripolyphosphate ( TPP) ,chitosan microspheres (CMs) loaded with adrenomedullin(ADM) were prepared by an emulsion-ionic cross-linking method .Then, Porous CMs/ nano-hydroxyapatite/ Poly(Lactide-co-Glycolide) composite was developed by thermally induced phase separation. Scanning electron microscope was used to analyze the characterization of the microspheres and scaffold. In vitro the drug release from microspheres and scaffold have been tested, also the hemolytic effect of the scaffold was tested to evaluate the toxicity of the biomaterial. The MG63 cell line and HUVEC cell line were cultured for testing the bioactivity of the scaffold loading ADM. The cell proliferation and differentiation was analyzed by MTT assay and ALP assay The result demonstrated the diameter of the microspere was well-distributed. The nHAC/PLGA/ADM scaffold showed a typical microstructure of polymeric foam prepared by thermally induced phase separation. The pore size ranged from 30μm to 220μm.and and the pores were interconnected.The hemolytic rate of the biomaterial was less than 5% ,which was in line with national standard of medical materials.The nHAC/PLGA/ADM and nHAC/PLGA scaffold could accelerate the proliferation of osteoblast-like cells and vascular endothelial cells, also were the stimuli for the differentiation of osteoblast-like cells.

Key words: Chitosan, microspheres, Poly(Lactide-co-Glycolide)(PLGA), nano-hydroxyapatite, adrenomedullin

TrendMD:

王林李春艳何盼付丽周延民陈学思. 载肾上腺髓质素微球复合PLGA/纳米羟基磷灰石支架材料的制备及其生物学活性评价. 高等学校化学学报, 2011, 32(7): 1622.

WANG Lin, LI Chun-Yan, HE Pan, FU Li, ZHOU Yan-Min*, CHEN Xue-Si. Preparation and Bioactivities of PLGA/Nano-hydroxyapatite Scaffold Containing Chitosan Microspheres for Controlled Delivery of Mutifuncational Peptide-adrenomedullin. Chem. J. Chinese Universities, 2011, 32(7): 1622.

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