蛋白质在导电聚甲基丙烯酰吡咯上的吸附

高等学校化学学报 ›› 2010, Vol. 31 ›› Issue (9): 1884.

蛋白质在导电聚甲基丙烯酰吡咯上的吸附

张治红, 闫立军, 梁燕, 冯孝中, 闫福丰, 郑先君, 王力臻

河南省表界面重点实验室, 郑州轻工业学院材料与化工学院, 郑州 450002

收稿日期:2009-11-27 出版日期:2010-09-10 发布日期:2010-09-10
通讯作者: 张治红, 女, 博士, 教授, 主要从事功能高分子材料作为生物分子吸附材料的研究. E-mail: mainzhh@yahoo.com.cn
基金资助:
国家自然科学基金 (批准号: 20704039)、河南省高校科技创新人才支持计划(批准号: 2010HASTIT023)和河南省国际科技合作计划(批准号: 094300510078)资助.

Adsorption of Protein on Conducting Poly(methacryloyl pyrrole)

ZHANG Zhi-Hong^*, YAN Li-Jun, LIANG Yan, FENG Xiao-Zhong, YAN Fu-Feng, ZHENG Xian-Jun, WANG Li-Zhen

Henan Provincial Key Laboratory of Surface & Interface Science, Zhengzhou University of Light Industry, Zhengzhou 450002, China

Received:2009-11-27 Online:2010-09-10 Published:2010-09-10
Contact: ZHANG Zhi-Hong. E-mail: mainzhh@yahoo.com.cn
Supported by:
国家自然科学基金 (批准号: 20704039)、河南省高校科技创新人才支持计划(批准号: 2010HASTIT023)和河南省国际科技合作计划(批准号: 094300510078)资助.

摘要/Abstract

摘要： 采用表面引发原子转移自由基聚合(SIATRP) 技术在硅片及金膜表面上制备导电聚丙烯酰吡咯(PMAP), 并对聚合物薄膜的化学结构等进行了表征. 采用表面等离子谐振生化分析仪(SPR)原位考察了牛血清白蛋白(BSA)在聚合物表面上的吸附行为, 通过改变缓冲液的pH值和BSA的浓度对蛋白质吸附的影响, 探讨了BSA在PMAP上的吸附机理. 采用电化学循环伏安测试考察了蛋白质吸附对于导电聚合物的电化学行为的影响. 采用原子力显微镜(AFM)考察了BSA在聚合物表面上吸附前后聚合物的表面形貌变化.

关键词: 表面引发原子转移自由基聚合, 牛血清白蛋白吸附, 表面等离子谐振仪, 聚吡咯

Abstract: Poly(methacryloyl pyrrole) films were prepared by surface initiated atom transfer radical polymerization(SIATRP) onto the surface of silicon and Au. The chemical structure, the surface wettability, the surface morphology and the electrochemical property were investgated. The adsorption behavior of BSA on the polymer surface was measured in situ by surface plasmon resonance spectroscopy. The adsorption mechanism of BSA could be revealed based on the effects of buffer pH values and BSA concentration on the protein adsorption. The change in the chemical property after BSA immobilization was characterized by cyclic voltammetry. The morphologies of PMAP before and after BSA adsorption were investigated by atom force microscopy. This study could supply the base of the preparation of the regular, uniform and sensitive films which are used in the field of biosensors.

Key words: Surface initiated atom transfer radical polymerization(SIATRP), Bovine serum albumin(BSA), Surfae plamson resonane spectrscopy(SPR), Polypyrrole

TrendMD:

张治红, 闫立军, 梁燕, 冯孝中, 闫福丰, 郑先君, 王力臻. 蛋白质在导电聚甲基丙烯酰吡咯上的吸附. 高等学校化学学报, 2010, 31(9): 1884.

ZHANG Zhi-Hong*, YAN Li-Jun, LIANG Yan, FENG Xiao-Zhong, YAN Fu-Feng, ZHENG Xian. Adsorption of Protein on Conducting Poly(methacryloyl pyrrole). Chem. J. Chinese Universities, 2010, 31(9): 1884.

参考文献

[1]Mikko S., Jouko K.. Langmuir[J], 2004, 20: 7794—7801 [2]Christian F. W., Maria M. S.. Langmuir[J], 2001, 17: 3006—3016 [3]Faucheux N., Schweiss R., Lutzow K., Werner C., Groth T.. Biomaterials[J], 2004, 25: 2721—2730 [4]Hashimoto H., Fujimara M., Hashimoto T., Kawai H.. Macromolecules[J], 1981, 14: 844—851 [5]Archambault J. G., Brash J. L.. Colloids and Surfaces B[J], 2004, 33: 111—120 [6]Schmitt A., Varoqui R., Uniyal S., Brash J. L., Pusineri C.. Colloid Interface Science[J], 1983, 92: 25—34 [7]Hashimoto T., Yamasaki K., Koizumi S., Hasegawa H.. Macromolecules[J], 1992, 26: 2895—2904 [8]Ide M., Mori T., Ichikawa K., Kitano H., Tanaka M., Mochizuki A., Oshiyama H., Mizuno W.. Langmuir[J], 2003, 19: 429—435 [9]Gerard M., Chaubey A., Malhotra B. D.. Biosens. Bioelectron[J], 2002, 17: 345—359 [10]Fernandes K. F., Lima C. S., Pinho H., Collins C. H.. Process Biochem.[J], 2003, 38: 1379—1384 [11]Crombrugghe A., Yunus S., Bertrand P.. Surface and Interface Analysis[J], 2008, 40: 404—414 [12]Peng H., Soeller C., Sejdic J. T.. Macromolecules[J], 2007, 40: 909—914 [13]Ramanavicius A., Kurilcik N., Jursenas S., Finkelsteinas A., Ramanaviciene A.. Biosens. Bioelectron[J], 2007, 23: 499—505 [14]Kwon N. H., Rahman M. A., Won M. S., Shim Y. B.. Analytical Chemistry[J], 2006, 78: 52—60 [15]Lenz D. M., Delamar M., Ferreira C. A.. Journal of Electroanalytical Chemistry[J], 2003, 540: 35—44 [16]Lee H., Yang H., Kwak J.. Electrochemistry Communications[J], 2002, 4: 128—133 [17]Tan H. G., Song Q. G., Niu X. H., Wang Z. Y., Gao W. F., Yan D. D.. J. Chem. Phys[J], 2009, 130: 214901-1—214901-7 [18]Yoon K. R., Ramaraj B., Lee S. M., Kim D. P.. Surface and Interface Analysis[J], 2008, 40: 1139—1143 [19]CHEN Yi-Wang (陈义旺), NIE Hua-Rong(聂华荣), SHEN Lie(谌烈), KANG En-Tang(康燕堂). Chem. J. Chinese Universities(高等学校化学学报)[J], 2005, 26(10): 1978—1980