碳纤维微电极表面负载含氧官能团及PDDA-纳米金粒子用于神经递质多巴胺的检测

doi:10.7503/cjcu20180034

高等学校化学学报 ›› 2018, Vol. 39 ›› Issue (8): 1656.doi: 10.7503/cjcu20180034

碳纤维微电极表面负载含氧官能团及PDDA-纳米金粒子用于神经递质多巴胺的检测

李元¹, 王婷婷¹, 李梅¹, 程寒^1,²()

1.中南民族大学药学院, 2.民族药学国家级实验教学示范中心, 武汉 430074

收稿日期:2018-01-09 出版日期:2018-08-10 发布日期:2018-06-27
作者简介:联系人简介: 程寒, 女, 博士, 副教授, 主要从事药物分析方面的研究. E-mail: chenghan@mail.scuec.edu.cn
基金资助:
国家自然科学基金(批准号: 21205144)、中南民族大学学术团队资助项目(批准号: XTZ15013)、中央高校基本科研业务费专项资金(批准号: CZY18036, CZZ16004)、中南民族大学大学生创新训练计划(批准号: 201710524029)和中南民族大学研究生创新基金计划(批准号: 2018SYCXJJ214)资助.

Determination of Dopamine Based on RO/Gold Nanoparticles-poly(dienedimethylammonium chloride) Modified Carbon Fiber Microelectrode^†

LI Yuan¹, WANG Tingting¹, LI Mei¹, CHENG Han^1,^2,^*()

1.School of Pharmaceutical Sciences, 2. National Demonstration Center for Experimental Ethnopharmacology Education, South-Central University for Nationalities, Wuhan 430074, China

Received:2018-01-09 Online:2018-08-10 Published:2018-06-27
Contact: CHENG Han E-mail:chenghan@mail.scuec.edu.cn
Supported by:
† Supported by the National Natural Science Foundation of China(No.21205144), the Academic Team Funding Program of South-Central University for Nationalities, China(No.XTZ15013), the Fundamental Research Funds for the Central Universities of China(Nos.CZY18036, CZZ16004), the Student Innovation and Venture Training Program of South-Central University for Nationalities, China(No.201710524029) and the Graduate Student Innovation Fund Program of South-Central University for Nationalities, China(No.2018SYCXJJ214).

摘要/Abstract

摘要：

将碳纤维微电极(CFME)置于超纯水中进行恒电位电沉积, 在其表面修饰含氧基团(RO); 再采用恒电位法在电极表面负载以聚二烯二甲基氯化铵(PDDA)为保护剂制备的纳米金粒子(AuNPs), 制得RO/AuNPs-PDDA/CFME修饰电极. 利用扫描电子显微镜对修饰前后碳纤维电极的表面形貌进行了表征, 探讨了修饰电极在多巴胺(DA)溶液中的电化学行为. 结果表明, 在20 mmol/L pH=7.0的Tris-HCl缓冲溶液中, 复合修饰膜RO/AuNPs-PDDA对DA具有显著的电催化活性. 在最优实验条件下, 采用差示脉冲伏安技术(DPV)对DA进行定量分析, DA的氧化峰电流与其浓度在1×10^-7~5×10^-5 mol/L范围内呈良好的线性关系, 相关系数R²=0.9912, 检出限达3.3×10^-8 mol/L(S/N=3). 该修饰方法重现性好, 电极稳定性佳, 制备方便, 可广泛应用于生物样品中DA的高灵敏分析.

关键词: 聚二烯二甲基氯化铵(PDDA), 功能化纳米金, 碳纤维微电极, 多巴胺

Abstract:

Carbon fiber microelectrode(CFME) was modified with oxygen-containing groups(RO) in pure water by potentiostatic method, then with poly(dially dimethyl ammonium chloride)(PDDA) functionalized gold nanoparticles(AuNPs) by electrochemical deposition method, as a result, the RO/AuNPs-PDDA/CFME was fabricated. The surface morphologies of different modified microelectrodes were characterized by means of scanning electron microscope(SEM), and the cyclic voltammetry behaviors of DA were investigated with modified electrodes. The research results showed that in pH 7.0, 20 mmol/L Tris-HCl buffer solution, the RO/AuNPs-PDDA/CFME exhibited excellent electrocatalytic effect toward dopamine(DA). Under the optimal experimental conditions, the oxidation peak current was proportional to the concentration of DA in the range of 1×10^-7—5×10^-5 mol/L with a correlation coefficient of 0.9912. The detection limit was 3.3×10^-8 mol/L(S/N=3). In brief, the RO/AuNPs-PDDA/CFME has the advantages of simple preparation, good reproducibility and high stability, thus, it is suitable for sensitive detection of DA.

Key words: Poly(dially dimethyl ammonium chloride)(PDDA), Functionalized AuNP, Carbon fiber microelectrode, Dopamine

中图分类号:

O657.1

TrendMD:

李元, 王婷婷, 李梅, 程寒. 碳纤维微电极表面负载含氧官能团及PDDA-纳米金粒子用于神经递质多巴胺的检测. 高等学校化学学报, 2018, 39(8): 1656.

LI Yuan, WANG Tingting, LI Mei, CHENG Han. Determination of Dopamine Based on RO/Gold Nanoparticles-poly(dienedimethylammonium chloride) Modified Carbon Fiber Microelectrode^†. Chem. J. Chinese Universities, 2018, 39(8): 1656.

图/表 6

Fig.1 SEM images of CFME before(inset) and after flame-burned(A), RO/CFME(B),PDDA-AuNPs/CFME(C) and RO/AuNPs-PDDA/CFME(D)Insets of (B)—(D): locally amplified electrode surface.

Fig.2 CV curves of 1.0×10-5 mol/L DA on different electrodes in 20 mmol/L pH=7.0 Tris-HCl buffer(A) and CV curves of potassium ferricyanide solutions with different pH values on PDDA-AuNPs/CFME(B)(A) a. CFME; b. RO/CFME; c. PDDA-AuNPs/CFME; d. RO/AuNPs-PDDA/CFME. Scan rate is 100 mV/s.

Fig.3 Effects of electrodeposition time on the oxidation peak currents of 1.0×10-5 mol/L DA using DPV measurements for RO/CFME(A) and RO/AuNPs-PDDA/CFME(B)

Fig.4 DPV curves of DA on the RO/AuNPs-PDDA/CFME in 20 mmol/L Tris-HCl(pH=7.0) containing different concentrations of DA(A) and plots of the peak current vs. concentration of DA(B)(A) c/(mol·L-1): a—h: 5×10-5, 4×10-5, 2×10-5, 1×10-5, 5×10-6, 1×10-6, 5×10-7, 1×10-7. (B) Error bars represent standard deviation of the mean from 5 detections. Initial voltage: -0.18 V, final voltage: 0.3 V, pulse width: 0.1 s, potential increase: 0.004 V, amplitude: 0.05 V.

Table 1 Comparison of DPV detection limits for DA*

Electrode	Detection limit/(mol·L^-1)	Ref.	Electrode	Detection limit/(mol·L^-1)	Ref.
a	6.0×10^-8	[21]	c	3.3×10^-8	This work
b	7.7×10^-8	[22]	d	2.3×10^-6	[23]

Table 2 Determination results of DA in mouse serum samples(n=5)*

Serum sample	c(Added)/(μmol·L^-1)	c(Found)/(μmol·L^-1)	Relative deviation(%)	Recovery(%)
1	10	9.53	2.5	95.3
2	20	20.82	1.2	104.1
3	30	29.56	0.9	98.5
4	40	41.90	2.3	104.8

参考文献 23

[1]	Duval F., Mokrani M.C., Monreal-Ortiz J. A., Fattah S., Champeval C., Schulz P., Psychoneuroendocrinology, 2007, 32(2), 876—888
[2]	Rogdaki M., Gudbrandsen M., Daly E., Schizophrenia Bull., 2017, 43(Suppl. 1), S75
[3]	Guo L., Zhang Y., Li Q., Anal. Sci., 2009, 25(12), 1451—1455
[4]	Liu Z., Chem. Pharm. Bull., 1993, 27(5), 1237—1244
[5]	Zhao J., Zhao L., Lan C., Zhao S., Sensor. Actuat.B: Chem., 2016, 223, 246—251
[6]	Xun Y., Cao Z., Song T.M., Lü C. Z., Liu F., He J. L., Yang R. H., Chem. J. Chinese Universities, 2016, 37(5), 835—843
	(寻艳, 曹忠, 宋天铭, 吕超志, 刘峰, 何婧琳, 杨荣华. 高等学校化学学报, 2016, 37(5), 835—843)
[7]	Honarvarfard E., Gamella M., Channaveerappa D.,Electroanal., 2017, 29(6), 1543—1553
[8]	Iacoban S., Mareci D., Bolat G., Munteanu C., Souto R.M., Metallurgical & Materials Transactions B, 2015, 46(2), 1011—1021
[9]	Luo Y., Jiang J., Zhou W., J. Mater. Chem. A, 2012, 22(17), 8634—8640
[10]	Özel R.E., Wallace K. N., Andreescu S., Anal. Chim. Acta, 2011, 695(1/2), 89—95
[11]	Chen R., Xiao H., Huang W., Tong H., Wang Z., Cheng J., Anal. Chem., 2003, 75(22), 6341—6345
[12]	Hu H.X., Hu S. R., Dong P. H., Tang Y. J., Hong K. J., Wang S. H., Chem. J. Chinese Universities, 2017, 38(7), 1171—1177
	(胡海霞, 胡世荣, 董培辉, 唐元军, 洪可俊, 王松华. 高等学校化学学报, 2017, 38(7), 1171—1177)
[13]	Kumar V.B., Borenstein A., Markovsky B., J. Phys. Chem. C, 2016, 120(25), 13406—13413
[14]	Chen J., He P., Bai H., Lei H., Zhang G., Dong F., Microchim. Acta, 2016, 183(12), 1—6
[15]	Sharma P., Radhakrishnan S., Jayaseelan S. S., Electroanal., 2016, 28(10), 2626—2632
[16]	Fan Y.J., Sha C. C., Tang W. Y., Li M., Wang Y., Cheng H., J. Central China Normal Univ.(Nat. Sci. ), 2015, 49(6), 904—906
	(范跃娟, 沙翠翠, 唐雯奕, 李梅, 王洋, 程寒. 华中师范大学学报(自然科学版), 2015, 49(6), 904—906)
[17]	Chen D., Cao Z., Liu F., Wu L., Xun Y., He J.L., Xiao Z. L., Chinese J. Anal. Chem., 2016, 44(10), 1593—1599
	(陈丹, 曹忠, 刘峰, 吴玲, 寻艳, 何婧琳, 肖忠良. 分析化学, 2016, 44(10), 1593—1599)
[18]	Wang Q., Zhang Q., Chen B., Lu X., Zhang S., Electrochem. Soc., 2015, 162(8), D320—D324
[19]	Takmakov P., Zachek M.K., Keithley R. B., Walsh P. L., Donley C., McCarty G. S., Wightman R. M, Anal. Chem., 2010, 82(5), 2020—2028
[20]	Ho C.C., Ding S. J., J. Mater. Sci.: Mater. Med., 2013, 24(10), 2381—2390
[21]	Zhu M., Zeng C., Ye J.,Electroanal., 2011, 23(4), 907—914
[22]	Łuczak T., Bełtowska-Brzezinska M., Microchim. Acta, 2011, 174(1/2), 19—30
[23]	Wang Q., Jiang N., Li N., Anal. Sci., 2001, 68(1), 77—85

碳纤维微电极表面负载含氧官能团及PDDA-纳米金粒子用于神经递质多巴胺的检测

Determination of Dopamine Based on RO/Gold Nanoparticles-poly(dienedimethylammonium chloride) Modified Carbon Fiber Microelectrode^†

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 6

参考文献 23

相关文章 15

编辑推荐

Metrics

本文评价

[1]	于鹏东, 关兴华, 王冬冬, 辛志荣, 石强, 殷敬华. 新型光、热双响应形状记忆聚合物的制备与性能[J]. 高等学校化学学报, 2022, 43(6): 20220085.
[2]	魏闯宇, 陈艳丽, 姜建壮. 基于乙硫基取代的三层酞菁铕二聚体修饰ITO电极构筑电化学多巴胺和尿酸传感器[J]. 高等学校化学学报, 2022, 43(1): 20210582.
[3]	王昱芃, 赵洋, 李茉涵, 史素青, 宫永宽. 多巴胺基多重相互作用协同构筑抗污-杀菌双重功能涂层[J]. 高等学校化学学报, 2021, 42(3): 811.
[4]	孙启睿, 赵楠, 刘树威, 辛华, 张皓, 张乐宁. 用于磁性靶向治疗肺纤维化的聚多巴胺包覆的Fe₃O₄/甲强龙/环磷酰胺复合超粒子[J]. 高等学校化学学报, 2021, 42(10): 3225.
[5]	王倩颖, 崔树勋. 通过自由基抑制剂研究聚多巴胺的形成机理[J]. 高等学校化学学报, 2020, 41(6): 1378.
[6]	谢璠,王亚芳,卓龙海,秦盼亮,宁逗逗,王丹妮,陆赵情. 高导热氮化硼/芳纶沉析复合薄膜的制备及性能[J]. 高等学校化学学报, 2020, 41(3): 582.
[7]	邬丰任,刘永佳,陆学民,朱邦尚. 聚多巴胺修饰金纳米花的可控制备及在光热治疗中的应用[J]. 高等学校化学学报, 2020, 41(3): 465.
[8]	王婷婷, 李元, 杨莉莉, 鲍昌昊, 程寒. 碳纤维微电极负载金纳米粒子用于芦荟多糖的在体动态监测[J]. 高等学校化学学报, 2020, 41(1): 87.
[9]	刘耘, 李婷, 汪洋, 东为富. 树莓状超疏水多孔复合棉纤维的制备及油水分离性能[J]. 高等学校化学学报, 2019, 40(8): 1775.
[10]	熊征蓉, 董莉, 刘向东, 杨宇明. PDA/PVDF紫外线屏蔽复合膜的制备及性能表征[J]. 高等学校化学学报, 2019, 40(4): 849.
[11]	杨书廷, 李倩慧, 范玉昌, 王秋娴, 杜婷, 岳红云. 双功能仿生膜修饰改性Li₄Ti₅O₁₂负极材料[J]. 高等学校化学学报, 2018, 39(11): 2513.
[12]	崔国廉, 但年华, 但卫华. 基于多巴胺的黏合水凝胶的制备及表征[J]. 高等学校化学学报, 2017, 38(2): 318.
[13]	于锡娟, 韩璐璐, 混旭. 基于铜离子修饰金纳米簇“关-开”型荧光探针检测多巴胺[J]. 高等学校化学学报, 2017, 38(12): 2169.
[14]	寻艳, 曹忠, 宋天铭, 吕超志, 刘峰, 何婧琳, 杨荣华. MWCNTs-rGO/PDDA-AuNPs复合膜修饰电极对莱克多巴胺的灵敏检测[J]. 高等学校化学学报, 2016, 37(5): 835.
[15]	刘树敏, 郑裕东, 李伟, 孙乙, 岳丽娜, 赵振江. 聚乙烯醇/酸化碳纳米管高亲水性三维孔电极膜的制备与电化学性能[J]. 高等学校化学学报, 2016, 37(2): 290.

碳纤维微电极表面负载含氧官能团及PDDA-纳米金粒子用于神经递质多巴胺的检测

Determination of Dopamine Based on RO/Gold Nanoparticles-poly(dienedimethylammonium chloride) Modified Carbon Fiber Microelectrode†

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 6

参考文献 23

相关文章 15

编辑推荐

Metrics

本文评价

Determination of Dopamine Based on RO/Gold Nanoparticles-poly(dienedimethylammonium chloride) Modified Carbon Fiber Microelectrode^†