Ag/Fe3O4/rGO纳米复合材料的制备及抗菌性能

doi:10.7503/cjcu20160753

摘要/Abstract

摘要：

以四水合氯化亚铁和硝酸银为原料, 硼氢化钠为还原剂, 氧化石墨烯(GO)为载体, 通过原位还原法制备了具有磁分离功能的银/四氧化三铁/还原氧化石墨烯(Ag/Fe₃O₄/rGO)纳米复合抗菌材料. 采用X射线粉末衍射仪(XRD)、 X射线光电子能谱仪(XPS)、透射电子显微镜(TEM)等对复合材料进行了表征. 结果显示, Fe₃O₄和Ag纳米颗粒均匀分布在rGO片层上. 复合材料的饱和磁化率(M_s)为40.5 A·m²·kg^-1, 表明其具有较强的磁性, 将其与菌液混合后, 在磁场作用下10 min即可吸附沉降完成磁分离. 以大肠杆菌(E. coli)和金黄色葡萄球菌(S. aureus)为实验菌株, 通过琼脂扩散法评价了复合材料的抗菌性能. 结果表明, 该复合材料具有良好的抗菌效果, 对E. coli和S. aureus的抑菌圈直径分别为18 mm和13 mm, 最低抑菌浓度值(MIC)分别为50 mg/L和80 mg/L, 最低杀菌浓度值(MBC)分别为30 mg/L和50 mg/L.

关键词: 氧化石墨烯, 纳米复合材料, 抗菌性能, 磁分离

Abstract:

Antibacterial sliver/ferroferric oxide/reduced graphene oxide(Ag/Fe₃O₄/rGO) nanocomposite with magnetic separation performance was prepared by in situ reduction method with FeCl₂·4H₂O and AgNO₃, using NaBH₄ as reducing agent and graphene oxide(GO) as the carrier. The nanocomposite was characterized by X-ray powder diffraction(XRPD), X-ray photoelectron spectroscopy(XPS) and transmission electron microscopy(TEM). The results showed that the Fe₃O₄ and Ag nanoparticles were uniformly distributed on the rGO sheets. Hysteresis loop test results indicated excellent magnetism of the nanocomposite, the saturation susceptibility(M_s) was 40.5 A·m²·kg^-1. Adsorption and settling of magnetic separation in the bacteria liquid could be completed within 10 min by adding Ag/Fe₃O₄/rGO in magnetic field. Selecting Escherichia coli(E. coli) and Staphylococcus aureus(S. aureus) as the experimental strains, the antibacterial properties of the composite was evaluated by the agar diffusion method, minimum inhibitory concentration(MIC) and minimum bactericidal concentration(MBC). Ag/Fe₃O₄/rGO showed strong antibacterial activities against E.coli and S.aureu. The antibacterial circle diameter was 18 mm and 13 mm, the MIC was 50 mg/L and 80 mg/L, and the MBC was 30 mg/L and 50 mg/L respectively.

Key words: Graphene oxide, Nanocomposite, Antibacterial, Magnetic separation

中图分类号:

O613
TB321

TrendMD:

杜文修, 杨娟, 桑玉祥, 张莉莉, 赵南, 徐凯, 程晓农. Ag/Fe₃O₄/rGO纳米复合材料的制备及抗菌性能. 高等学校化学学报, 2017, 38(3): 346.

DU Wenxiu, YANG Juan, SANG Yuxiang, ZHANG Lili, ZHAO Nan, XU Kai, CHENG Xiaonong. Preparation and Antibacterial Properties of Ag/Fe₃O₄/rGO Nanocomposite^†. Chem. J. Chinese Universities, 2017, 38(3): 346.

图/表 11

Scheme 1 Preparation process and adsorption sedimentation of Ag/Fe3O4/rGO nanocomposite

Fig.1 XRD patterns of Ag/Fe3O4/rGO nanocomposite(A) and thermogravimetric curves of Fe3O4/rGO nanocomposite(B) The inset shows the XRD pattern of Fe3O4/rGO.

Fig.2 C1s(A), O1s(B), Ag3d(C) and Fe2p(D) XPS spectra of Ag/Fe3O4/rGO nanocomposite

Fig.3 TEM images of Fe3O4/rGO(A), Ag/Fe3O4/rGO(B), HRTEM image of Ag/Fe3O4/rGO(C) and STEM image of Ag/Fe3O4/rGO nanocomposite(D)

Fig.4 Magnetic hysteresis loop of Ag/Fe3O4/rGO nanocomposite The inset shows the magnetic separation of Ag/Fe3O4/rGO nanocomposite.

Fig.5 Adsorption sedimentation images of E. coli and S. aureus treated by Ag/Fe3O4/rGO nanocomposite for 1 min(A), 5 min(B) and 10 min(C), different bacterial liquid without magnetic field(D) and in magnetic field(E) for 1 h E: E. coli; S: S. aureus; N: Ag/Fe3O4/rGO nanocomposite; r: rGO.

Fig.6 Antibacterial activity of Fe3O4/rGO(500 mg/L), Ag(5 mg/L) and Ag/Fe3O4/rGO(500 mg/L) nanocomposite against E. coli in agar-well diffusion assay

Fig.7 Antibacterial activity of Ag/Fe3O4/rGO nanocomposite against E. coli(A) and S. aureus(B) in agar-well diffusion assay

Fig.8 Bacterial growth curves of E. coli(A) and S. aureu(B) streated by Ag/Fe3O4/rGO nanocomposite

Fig.9 Photographs of E. coli(A—C) and S. aureus(D—F) colonies on an agar plate treated by Ag/Fe3O4/rGO nanocomposite for 1 h(A, D), 2 h(B, E) and 3 h(C, F) (A)—(C) E. coli+30 mg/L Ag/Fe3O4/rGO; (D)—(F) S. aureus+50 mg/L Ag/Fe3O4/rGO.

Fig.10 Cell viability of human HepG2 cell line in the presence of different materials

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Ag/Fe₃O₄/rGO纳米复合材料的制备及抗菌性能

Preparation and Antibacterial Properties of Ag/Fe₃O₄/rGO Nanocomposite^†

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