高等学校化学学报

高等学校化学学报

• 研究快报 • 上一篇    下一篇

PPESO3监测微波辅助酶解蛋白的进程

郇延富1, 张志权1, 费强1, 冯国栋1,2, 牟颖2,3   

    1. 吉林大学化学学院, 长春 130012;
    2. 吉林大学分子酶学工程教育部重点实验室, 长春 130021;
    3. 浙江大学工业控制技术国家重点实验室, 智能系统与控制研究所, 分析仪器研究中心, 杭州 310058
  • 收稿日期:2008-08-21 修回日期:1900-01-01 出版日期:2009-01-10 发布日期:2009-01-10
  • 通讯作者: 冯国栋, 牟颖

PPESO3 Monitoring the Proress of Microwave-enhanced Enzymatic Digestion on Protein

HUAN Yan-Fu1, ZHANG Zhi-Quan1, FEI Qiang1, FENG Guo-Dong1,2*, MU Ying2,3*   

    1. College of Chemistry, Jilin University, Changchun 130012, China;
    2. Key Laboratory for Molecular Enzymology and Engineering of the Ministry of Education, Jilin University, Changchun 130021, China;
    3. Research Center for Analytical Instrumentation, Institute of Cyber-systems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou 310058, China
  • Received:2008-08-21 Revised:1900-01-01 Online:2009-01-10 Published:2009-01-10
  • Contact: FENG Guo-Dong, MU Ying

PDF (PC)

摘要： 本实验将胰蛋白酶固定在荧光聚合物PPESO3上, 固定后的胰蛋白酶在酶解蛋白过程中通过监测荧光聚合物荧光强度的变化, 从而实现监测蛋白酶解过程.

关键词: 荧光共轭聚合物, 微波辅助酶解, 蛋白质

Abstract: The mini microwave resonance cavity was used to accelerate proteolytic cleavage of protein. A fluorescent polymer PPESO3 as a fluorescent indicator was used to monitor proteolytic cleavage of protein in the microwave assisted protein digestion process. In this paper, the long-term stability of the PPESO3 and the PPESO3 mixed with trypsin was studied, and the detection of proteolytic cleavage of bovine serum albumin(BSA) was obtained satisfactory results. The PPESO3 was provided a novel, simple, quick determination to set the optimization parameter of the microwave-assisted protein hydrolysis. Meanwhile, the fluorescent detection devices can be integrated into the microwave assisted protein digestion system, for the development of the mini Low-power microwave protein proteolytic system would provide a very good idea.

Key words: Fluorescent conjugated polymer, Microwave-accelerated enzymatic digestion, Protein

中图分类号: 

TrendMD: